Compound A Increases Cell Infiltration in Target Organs of Acute Graft-versus-Host Disease (aGVHD) in a Mouse Model
Abstract
:1. Introduction
2. Results
2.1. Influence of CpdA on Proliferation and Inflammation In Vitro
2.2. Effect of CpdA on Allo-BMT In Vivo
2.3. Cytokine Expression and Pathology Score
2.4. Analysis of Cell Suspensions of Target Organs
2.5. Expression of Chemokines and Chemokine Receptors after MLR
3. Discussion
4. Materials and Methods
4.1. Transplantation Procedure
4.2. Semiquantitative Histopathology of the Liver, Lung, Gastrointestinal Tract, and Skin
4.3. Serum Cytokine Analysis after BMT
4.4. Single-Cell Suspensions and Flow Cytometry Analysis
4.5. T Cell Proliferation and In Vitro Mixed Lymphocyte Reaction (MLR)
4.6. Determination of mRNA Expression Using Real-Time PCR
4.7. Statistical Methods
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Sample Availability
References
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β-Act-sense | GCT GTC CCT GTA TGC CTC TG |
β-Act-antisense | GTG GTG GTG AAG CTG TAG CC |
CCL3(MIP-1α)-sense | TGC CTG CTG CTT CTC CTA CA |
CCL3(MIP-1α)-antisense | TGG ACC CAG GTC TCT TTG GA |
CCL4(MIP-1β)-sense | CCA GGG TTC TCA GCA CCA A |
CCL4(MIP-1β)-antisense | GCT CAC TGG GGT TAG CAC AGA |
CCL5 (RANTES) sense | CTCACCATATGGCTCGGACA |
CCL5 (RANTES) antisense | CTTCTCTGGGTTGGCACACA |
CXCL1(KC) sense | GCCTATCGCCAATGAGCTG |
CXCL1(KC) antisense | CTGAACCAAGGGAGCTTCAGG |
CXCL9(MIG)-sense | TGG GCA TCA TCT TCC TGG AG |
CXCL9(MIG)-antisense | CCG GAT CTA GGC AGG TTT GA |
CXCL10(IP-10)-sense | CCT CAT CCT GCT GGG TCT G |
CXCL10(IP-10)-antisense | CTC AAC ACG TGG GCA GGA |
CXCL11(I-TAC)-sense | CGG GAT GAA AGC CGT CAA |
CXCL11(I-TAC)-antisense | TAT GAG GCG AGC TTG CTT GG |
CCL22 (MDC) sense | TGGCTCTCGTCCTTCTTGCT |
CCL22 (MDC) antisense | AGGCTTGCGGCAGGATTT |
IFN γ-sens | CAG GCC ATC AGC AAC AAC AT |
IFN γ-antisens | CGC TTC CTG AGG CTG GAT T |
TNF-sens | TAC GTG CTC CTC ACC CAC AC |
TNF-antisens | AGT TGG TCC CCC TTC TCC AG |
CCR1 sense | TTCCTCCTCTGGACCCCCTA |
CCR1 antisense | TTGAAACAGCTGCCGAAGGT |
CCR2 sense | CCACACCCTGTTTCGCTGTA |
CCR2 antisense | TGCATGGCCTGGTCTAAGTG |
CCR3 sense | TTTGGACCCCGTACAACCTG |
CCR3 antisense | TTTCCGGAACCTCTCACCAA |
CCR5 sense | CAGGGCTGTGAGGCTCATCT |
CCR5 antisense | GGCAGCAGTGTGTCATTCCA |
CCR7 sense | CCAATAGCAGCTGCGAAACC |
CCR7 antisense | GCAGCCCAAGTCCTTGAAGA |
CCR9 sense | GGTCACCTTGGGGTTTTTCC |
CCR9 antisense | TAAGCGTCAACAGCCTGCAC |
CCR10 sense | CCAGCAAGCGCAAGGATCTA |
CCR10 antisense | AGCAGGAAGAAAGGCGGAGT |
CXCR1 sense | TGGGGGTGATCTTTGCTGTT |
CXCR1 antisense | TTTGGCCAACGAAGGCATAG |
CXCR2 sense | CATCTTCGCTGTCGTCCTTGT |
CXCR2 antisense | GCTGTGGAGGAAGCCAAGAA |
CXCR3 sense | TAGTGGTGGTGGCAGCCTTT |
CXCR3 antisense | AGGCATAGAGCAGCGGATTG |
CXCR6 sense | CTTTCGGGCTTGCCTTAACC |
CXCR6 antisense | CATTGTGGGAGGCAGAACAA |
IL23a sense | GCCCCGTATCCAGTGTGAAG |
IL23a antisense | CTGGGCATCTGTTGGGTCTC |
IL17a sense | TCCACGTCACCCTGGACTCT |
IL17a antisense | CCCACCAGCATCTTCTCGAC |
IL2 sense | GGACCTCTGCGGCATGTT |
IL2 antisense | TCTCCTCAGAAAGTCCACCACA |
IL4 sense | GATGTGCCAAACGTCCTCAC |
IL4 antisense | AAGCCCGAAAGAGTCTCTGC |
IL10 sense | GGGTTGCCAAGCCTTATCG |
IL10 antisense | TGCTCCACTGCCTTGCTCT |
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Bouazzaoui, A.; Abdellatif, A.A.H.; Al-Allaf, F.A.; Bogari, N.M.; Taher, M.M.; Athar, M.; Schubert, T.; Habeebullah, T.M.; Qari, S.H. Compound A Increases Cell Infiltration in Target Organs of Acute Graft-versus-Host Disease (aGVHD) in a Mouse Model. Molecules 2021, 26, 4237. https://doi.org/10.3390/molecules26144237
Bouazzaoui A, Abdellatif AAH, Al-Allaf FA, Bogari NM, Taher MM, Athar M, Schubert T, Habeebullah TM, Qari SH. Compound A Increases Cell Infiltration in Target Organs of Acute Graft-versus-Host Disease (aGVHD) in a Mouse Model. Molecules. 2021; 26(14):4237. https://doi.org/10.3390/molecules26144237
Chicago/Turabian StyleBouazzaoui, Abdellatif, Ahmed A. H. Abdellatif, Faisal A. Al-Allaf, Neda M. Bogari, Mohiuddin M. Taher, Mohammad Athar, Thomas Schubert, Turki M. Habeebullah, and Sameer H. Qari. 2021. "Compound A Increases Cell Infiltration in Target Organs of Acute Graft-versus-Host Disease (aGVHD) in a Mouse Model" Molecules 26, no. 14: 4237. https://doi.org/10.3390/molecules26144237