In Vitro Anti-Tubulin Activity on MCF10A Cell Line and In Silico Rigid/Semiflexible-Residues Docking, of Two Lignans from Bursera Fagaroides var. Fagaroides
Abstract
:1. Introduction
2. Results
2.1. Microscopy Anti-Tubulin Test
2.2. Topoisomerase II Inhibition Assay
2.3. Rigid- and Semiflexible-Residues Molecular Docking
3. Discussion
4. Materials and Methods
4.1. Microscopy Anti-Tubulin Test
4.2. Microtubules Semi-Quantitation
4.3. Topoisomerase II Inhibition Assay
4.4. Flexible- and Rigid-Residues Molecular Docking
4.5. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Sample Availability
References
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Rigid Residues | Flexible Residues | |||
---|---|---|---|---|
Compound | ΔGb (Kcal/Mol) | KD (M) | ΔGb (Kcal/Mol) | KD (M) |
POD | −8.6 ± 0.00 | 4.96 × 10−7 ± 0.00 | −8.6 ± 1.15 | 1.44 × 10−5 ± 5.05 × 10−5 |
1 | −6.9 ± 0.03 | 8.53 × 10−6 ± 5.08 × 10−7 | −3.6 ± 1.05 | 0.011 ± 0.03 |
2 | −9.4 ± 0.00 | 1.28 × 10−7 ± 0.00 | −10.8 ± 0.81 | 3.10 × 10−8 ± 5.97 × 10−8 |
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Rodríguez-López, V.; Millán-Pacheco, C.; González-Christen, J.; Anaya-Ruíz, M.; Peña-Morán, O.A. In Vitro Anti-Tubulin Activity on MCF10A Cell Line and In Silico Rigid/Semiflexible-Residues Docking, of Two Lignans from Bursera Fagaroides var. Fagaroides. Molecules 2021, 26, 6155. https://doi.org/10.3390/molecules26206155
Rodríguez-López V, Millán-Pacheco C, González-Christen J, Anaya-Ruíz M, Peña-Morán OA. In Vitro Anti-Tubulin Activity on MCF10A Cell Line and In Silico Rigid/Semiflexible-Residues Docking, of Two Lignans from Bursera Fagaroides var. Fagaroides. Molecules. 2021; 26(20):6155. https://doi.org/10.3390/molecules26206155
Chicago/Turabian StyleRodríguez-López, Verónica, César Millán-Pacheco, Judith González-Christen, Maricruz Anaya-Ruíz, and Omar Aristeo Peña-Morán. 2021. "In Vitro Anti-Tubulin Activity on MCF10A Cell Line and In Silico Rigid/Semiflexible-Residues Docking, of Two Lignans from Bursera Fagaroides var. Fagaroides" Molecules 26, no. 20: 6155. https://doi.org/10.3390/molecules26206155