Inhibition of Cytosolic Phospholipase A2α Induces Apoptosis in Multiple Myeloma Cells
Abstract
:1. Introduction
2. Results
2.1. cPLA2α Is Overexpressed in Cancers and Cancer Cell Lines from Different Tissue Origins
2.2. Hematological Cancer Cells Are Sensitive to cPLA2α Inhibition
2.3. cPLA2α Is Overexpressed in Hematological Cancers including Multiple Myeloma
2.4. Inhibition of cPLA2α Reduces Cell Viability of Multiple Myeloma Cells
2.5. cPLA2α Inhibitors Induce Apoptosis in Caspase-3 Dependent Pathways
3. Discussion
4. Materials and Methods
4.1. Materials
4.2. Maintenance of Multiple Myeloma Cell Lines
4.3. Viability Screening of Oncolines Cancer Cell Panel
4.4. Cell Viability Assays
4.5. Annexin V-FITC and Propidium Iodide Apoptosis Assay
4.6. Caspase-3/7 Activity Assay
4.7. Western Blot
4.8. Statistical Analysis
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Sample Availability
References
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Mahammad, N.; Ashcroft, F.J.; Feuerherm, A.J.; Elsaadi, S.; Vandsemb, E.N.; Børset, M.; Johansen, B. Inhibition of Cytosolic Phospholipase A2α Induces Apoptosis in Multiple Myeloma Cells. Molecules 2021, 26, 7447. https://doi.org/10.3390/molecules26247447
Mahammad N, Ashcroft FJ, Feuerherm AJ, Elsaadi S, Vandsemb EN, Børset M, Johansen B. Inhibition of Cytosolic Phospholipase A2α Induces Apoptosis in Multiple Myeloma Cells. Molecules. 2021; 26(24):7447. https://doi.org/10.3390/molecules26247447
Chicago/Turabian StyleMahammad, Nur, Felicity J. Ashcroft, Astrid J. Feuerherm, Samah Elsaadi, Esten N. Vandsemb, Magne Børset, and Berit Johansen. 2021. "Inhibition of Cytosolic Phospholipase A2α Induces Apoptosis in Multiple Myeloma Cells" Molecules 26, no. 24: 7447. https://doi.org/10.3390/molecules26247447