In Vivo Biological Behavior of Polymer Scaffolds of Natural Origin in the Bone Repair Process
Abstract
:1. Introduction
2. Results
2.1. Radiological and Macroscopic Analysis
2.2. Micro CT Analysis: Three-Dimensional, Two-Dimensional Sections and Quantitative Evaluation
2.3. Confocal Laser Scanning Microscopy Analysis
2.4. Mineralization Analysis of New Bone Formed by the Von Kossa’s Method
2.5. Birefringence Analysis of Collagen Fibers
2.6. Histological Evaluation of Neoformed Bone and Histomorphometric Evaluation
3. Discussion
4. Materials and Methods
4.1. Elastin Matrix Derived from Bovine Auricular Cartilage
4.2. Collagen Matrices Derived from Porcine Intestinal Serosa
4.3. Experimental Design
4.4. Bone Defect Surgeries
4.5. Sequential Fluorescent Labeling
4.6. Histologic Preparation for Confocal Laser Scanning Microscopy Analysis
4.7. Histologic Preparation and Von Kossa’s Staining Method
4.8. Macroscopic and Radiological Analysis
4.9. Micro-CT Scan (μ-CT)
4.10. Histological Procedures–Masson’s Trichrome Staining and Picrosirius Red
4.11. Histomorphometric Evaluation
4.12. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Sample Availability
References
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Cunha, F.B.; Pomini, K.T.; Plepis, A.M.d.G.; Martins, V.d.C.A.; Machado, E.G.; de Moraes, R.; Munhoz, M.d.A.e.S.; Machado, M.V.R.; Duarte, M.A.H.; Alcalde, M.P.; et al. In Vivo Biological Behavior of Polymer Scaffolds of Natural Origin in the Bone Repair Process. Molecules 2021, 26, 1598. https://doi.org/10.3390/molecules26061598
Cunha FB, Pomini KT, Plepis AMdG, Martins VdCA, Machado EG, de Moraes R, Munhoz MdAeS, Machado MVR, Duarte MAH, Alcalde MP, et al. In Vivo Biological Behavior of Polymer Scaffolds of Natural Origin in the Bone Repair Process. Molecules. 2021; 26(6):1598. https://doi.org/10.3390/molecules26061598
Chicago/Turabian StyleCunha, Fernando Bento, Karina Torres Pomini, Ana Maria de Guzzi Plepis, Virgínia da Conceição Amaro Martins, Eduardo Gomes Machado, Renato de Moraes, Marcelo de Azevedo e Souza Munhoz, Michela Vanessa Ribeiro Machado, Marco Antonio Hungaro Duarte, Murilo Priori Alcalde, and et al. 2021. "In Vivo Biological Behavior of Polymer Scaffolds of Natural Origin in the Bone Repair Process" Molecules 26, no. 6: 1598. https://doi.org/10.3390/molecules26061598