Polymer–Colloid Complexes Based on Cationic Imidazolium Amphiphile, Polyacrylic Acid and DNA Decamer
Abstract
:1. Introduction
2. Results and Discussion
2.1. Mixed Systems Based on IA-12 and Polyacrylic Acid
2.2. Amphiphile/DNA Decamer Interactions
2.3. Membranotropic Properties and Cell Penetration
3. Materials and Methods
3.1. Chemicals
3.2. Preparation of Samples
3.3. Methods
3.3.1. Tensiometry
3.3.2. Conductometry
3.3.3. Fluorescence Spectroscopy
3.3.4. Dynamic and Electrophoretic Light Scattering
3.3.5. Electrophoretic Analysis
3.3.6. Turbidimetry
3.3.7. Fluorescence Microscopy
3.3.8. Flow Cytometry Assay
3.3.9. In Vitro Cytotoxicity Assay
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Sample Availability
References
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CPAA, mM | Aggregation Thresholds (mM) | |||
---|---|---|---|---|
Tensiometry | Conductometry | Fluorimetry | ||
CAC1 | CAC2 | |||
0 | 9 [43] | - | 10 [43] | 9 [43] |
1 | 0.05 | 10.0 | 5 | 0.05 |
3 | 0.08 | 9.0 | 4 | 0.05 |
5 | 0.10 | 8.0 | 3.5 | 0.07 |
IC50 µM | |||
---|---|---|---|
M-HeLa | Chang Liver | ||
IA-12 | IA-12/oNu | IA-12 | IA-12/oNu |
39 ± 0.1 | 1.1 ± 0.08 | 17.0 ± 0.1 | 21.3 ± 1.8 |
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Kuznetsova, D.A.; Gabdrakhmanov, D.R.; Kuznetsov, D.M.; Lukashenko, S.S.; Zakharov, V.M.; Sapunova, A.S.; Amerhanova, S.K.; Lyubina, A.P.; Voloshina, A.D.; Salakhieva, D.V.; et al. Polymer–Colloid Complexes Based on Cationic Imidazolium Amphiphile, Polyacrylic Acid and DNA Decamer. Molecules 2021, 26, 2363. https://doi.org/10.3390/molecules26082363
Kuznetsova DA, Gabdrakhmanov DR, Kuznetsov DM, Lukashenko SS, Zakharov VM, Sapunova AS, Amerhanova SK, Lyubina AP, Voloshina AD, Salakhieva DV, et al. Polymer–Colloid Complexes Based on Cationic Imidazolium Amphiphile, Polyacrylic Acid and DNA Decamer. Molecules. 2021; 26(8):2363. https://doi.org/10.3390/molecules26082363
Chicago/Turabian StyleKuznetsova, Darya A., Dinar R. Gabdrakhmanov, Denis M. Kuznetsov, Svetlana S. Lukashenko, Valery M. Zakharov, Anastasiia S. Sapunova, Syumbelya K. Amerhanova, Anna P. Lyubina, Alexandra D. Voloshina, Diana V. Salakhieva, and et al. 2021. "Polymer–Colloid Complexes Based on Cationic Imidazolium Amphiphile, Polyacrylic Acid and DNA Decamer" Molecules 26, no. 8: 2363. https://doi.org/10.3390/molecules26082363