The Effect of Sericin from Various Extraction Methods on Cell Viability and Collagen Production
Abstract
:1. Introduction
2. Results and Discussion
2.1. Molecular Weight of SS
2.2. Particle Size and Zeta Potential Measurement
2.3. Amino Acid Analysis
2.4. Cytotoxicity of SS Solution
2.5. Adherence of L929 Mouse Fibroblast Cell Line to SS Films
2.6. Determination of Soluble Collagen Production Induced by SS
3. Experimental Section
3.1. Materials
3.1.1. Silkworm Cocoons
3.1.2. Fibroblast Cell Culture
3.1.3. Preparation of SS Powder Using a High Temperature and High Pressure Degumming Technique (Heat-Degraded SS Powder)
3.1.4. Preparation of SS by Citric Acid and Sodium Carbonate Solution (Acid-Degraded and Alkali-Degraded SS Powders)
3.1.5. Preparation of SS by Urea Solution
3.2. Methods
3.2.1. Molecular Weight Determination
3.2.2. Particle Size and Zeta Potential Measurement
3.2.3. Amino Acid Analysis
3.2.4. Cytotoxicity of SS Solution
3.2.5. Adherence of L929 Mouse Fibroblast Cell Line on SS Films
3.2.6. Determination of Soluble Collagen Production Induced by SS
4. Conclusions
Acknowledgments
References and Notes
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Extraction method | Zeta potential (mV) | Mean size (nm) |
---|---|---|
Heat | −20.69 ± 2.14 | 110.42 ± 35.07 |
Acid | −32.12 ± 5.26 | 23.80 ± 16.07 |
Alkaline | −15.87 ± 2.89 | 824.42 ± 86.67 |
Urea | −68.36 ± 5.67 | 4.62 ± 2.44 |
Amino acid | Extraction method of SS | |||
---|---|---|---|---|
Heat | Urea | Acid | Alkaline | |
Asp | 15.64 | 18.31 | 15.93 | 19.88 |
Ser | 33.63 | 31.27 | 31.86 | 30.01 |
Glu | 4.61 | 5.27 | 5.75 | 5.93 |
Gly | 15.03 | 11.23 | 10.49 | 11.01 |
His | 1.06 | 3.26 | 2.47 | 1.72 |
Arg | 2.87 | 5.41 | 4.92 | 4.92 |
Thr | 8.16 | 8.36 | 8.51 | 6.49 |
Ala | 4.10 | 4.33 | 3.72 | 4.21 |
Pro | 0.54 | 1.46 | 0.78 | 1.24 |
Cys | 0.54 | 0.39 | 0.53 | 0.23 |
Tyr | 3.45 | 0.36 | 5.56 | 5.24 |
Val | 2.88 | 2.96 | 2.95 | 2.94 |
Met | 3.39 | 0.12 | 0.06 | 0.15 |
Lys | 2.35 | 3.14 | 3.48 | 2.89 |
Ile | 0.56 | 0.96 | 0.87 | 0.75 |
Leu | 1.00 | 1.58 | 1.43 | 1.56 |
Phe | 0.28 | 0.60 | 0.71 | 0.81 |
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Aramwit, P.; Kanokpanont, S.; Nakpheng, T.; Srichana, T. The Effect of Sericin from Various Extraction Methods on Cell Viability and Collagen Production. Int. J. Mol. Sci. 2010, 11, 2200-2211. https://doi.org/10.3390/ijms11052200
Aramwit P, Kanokpanont S, Nakpheng T, Srichana T. The Effect of Sericin from Various Extraction Methods on Cell Viability and Collagen Production. International Journal of Molecular Sciences. 2010; 11(5):2200-2211. https://doi.org/10.3390/ijms11052200
Chicago/Turabian StyleAramwit, Pornanong, Sorada Kanokpanont, Titpawan Nakpheng, and Teerapol Srichana. 2010. "The Effect of Sericin from Various Extraction Methods on Cell Viability and Collagen Production" International Journal of Molecular Sciences 11, no. 5: 2200-2211. https://doi.org/10.3390/ijms11052200