Antioxidant Activities of Fractions of Polymeric Procyanidins from Stem Bark of Acacia confusa
Abstract
:1. Introduction
2. Results and Discussion
2.1. Antioxidant Activities of Fractions F1-F11
2.2. MALDI-TOF MS Analysis
2.3. Thiolysis of Fractions F2–F11
3. Experimental Section
3.1. Chemicals and Plant Materials
3.2. Extraction and Fractionation of Procyanidin Polymers
3.3. DPPH Radical Scavenging Activity
3.4. Ferric Reducing/Antioxidant Power (FRAP) Assay
3.5. MALDI-TOF MS Analysis
3.6. Thiolysis Reaction and HPLC Analysis
3.7. Statistical Analysis
4. Conclusions
Acknowledgments
References
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Fractions | Antioxidant Activity | |
---|---|---|
IC50/DPPH (μg/mL) a | FRAP (mmol AAE/g) b | |
F1 | 450.24 ± 5.21 a | 1.33 ± 0.06 g |
F2 | 136.38 ± 4.01 b | 4.20 ± 0.09 e |
F3 | 116.64 ± 1.83 d | 5.55 ± 0.12 c |
F4 | 99.12 ± 0.77 e | 6.09 ± 0.06 b |
F5 | 90.74 ± 0.86 g | 6.13 ± 0.02 b |
F6 | 93.88 ± 0.44 f | 5.34 ± 0.06 d |
F7 | 89.84 ± 0.74 g | 5.52 ± 0.07 c |
F8 | 87.42 ± 1.82 g | 5.50 ± 0.06 c |
F9 | 81.91 ± 0.60 h | 6.33 ± 0.04 a |
F10 | 115.23 ± 0.94 d | 4.29 ± 0.05 e |
F11 | 129.72 ± 1.77 c | 3.97 ± 0.04 f |
Ascorbic acid | 101.96 ± 1.84 e | - |
BHA | 116.91 ± 0.97 d | 5.18 ± 0.11 d |
Polymer | Observed [M+Cs]+ | |||||||
---|---|---|---|---|---|---|---|---|
F2 | F3 | F4 | F5 | F6 | F7 | F8 | F9 | |
DP2 | -- | 711.21 | 711.13 | -- | -- | -- | -- | -- |
DP3 | 999.25 | 999.29 | 999.20 | 999.24 | 999.26 | 999.43 | 999.37 | 999.36 |
DP4 | -- | 1287.41 | 1287.26 | 1287.30 | 1287.33 | 1287.40 | 1287.33 | 1287.38 |
DP5 | -- | -- | 1575.35 | 1575.36 | 1575.38 | 1575.53 | 1575.51 | 1575.62 |
DP6 | -- | -- | -- | 1864.46 | 1864.45 | 1864.63 | 1863.59 | 1863.57 |
DP7 | -- | -- | -- | -- | 2152.54 | 2152.70 | 2152.60 | 2152.60 |
DP8 | -- | -- | -- | -- | 2440.63 | 2440.78 | 2440.70 | 2440.63 |
DP9 | -- | -- | -- | -- | -- | 2728.87 | 2728.76 | 2728.71 |
DP10 | -- | -- | -- | -- | -- | 3016.93 | 3017.69 | 3017.10 |
DP11 | -- | -- | -- | -- | -- | 3304.86 | 3305.39 | 3305.81 |
DP12 | -- | -- | -- | -- | -- | -- | 3593.99 | 3593.81 |
DP13 | -- | -- | -- | -- | -- | -- | 3881.56 | 3881.59 |
DP14 | -- | -- | -- | -- | -- | -- | -- | 4169.72 |
DP15 | -- | -- | -- | -- | -- | -- | -- | 4457.63 |
Fractions | mDP by Thiolysis | Polymer Range in MALDI | Most Intense Polymer in MALDI |
---|---|---|---|
F2 | 1.68 ± 0.05 j | 3 | 3 |
F3 | 2.13 ± 0.16 i | 2–4 | 3 |
F4 | 2.71 ± 0.06 h | 2–5 | 2 |
F5 | 5.46 ± 0.09 g | 3–6 | 4 |
F6 | 7.37 ± 0.08 f | 3–8 | 5 |
F7 | 9.49 ± 0.11 e | 3–11 | 6 |
F8 | 9.80 ± 0.09 d | 3–13 | 8 |
F9 | 10.65 ± 0.13 c | 3–15 | 9 |
F10 | 15.44 ± 0.06 b | - | - |
F11 | 17.31 ± 0.16 a | - | - |
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Wei, S.-D.; Zhou, H.-C.; Lin, Y.-M. Antioxidant Activities of Fractions of Polymeric Procyanidins from Stem Bark of Acacia confusa. Int. J. Mol. Sci. 2011, 12, 1146-1160. https://doi.org/10.3390/ijms12021146
Wei S-D, Zhou H-C, Lin Y-M. Antioxidant Activities of Fractions of Polymeric Procyanidins from Stem Bark of Acacia confusa. International Journal of Molecular Sciences. 2011; 12(2):1146-1160. https://doi.org/10.3390/ijms12021146
Chicago/Turabian StyleWei, Shu-Dong, Hai-Chao Zhou, and Yi-Ming Lin. 2011. "Antioxidant Activities of Fractions of Polymeric Procyanidins from Stem Bark of Acacia confusa" International Journal of Molecular Sciences 12, no. 2: 1146-1160. https://doi.org/10.3390/ijms12021146