Isolation and Characterization of Microsatellite Loci for Hibiscus aridicola (Malvaceae), an Endangered Plant Endemic to the Dry-Hot Valleys of Jinsha River in Southwest China
Abstract
:1. Introduction
2. Results and Discussion
3. Experimental Section
4. Conclusions
Acknowledgements
References
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Locus | Primer Sequence (5′-3′) | Repeat motif | NA | Size (bp) | Ta (°C) | Gene Bank Accession No. |
---|---|---|---|---|---|---|
HA-1 | F: TTGAACATAAACAAGCGG R: AAAACAAGTTGGGGAGG | (GT)14 | 2 | 180–200 | 56 | JN167557 |
HA-2 | F: CTGAATGCCAGAATGACT R: CAGGCGAAAGAGGAAGAT | (AG)21 | 5 | 410–445 | 60 | JN167558 |
HA-3 | F: ATCATTATCATCTTCGTTTC R: AAGGGACCAAAGTCTCAA | (CT)15 | 2 | 141–154 | 59 | JN167559 |
HA-4 | F: CACCAAATCCTGGAGAAG R: GCAAACGAGAATAATCAAAA | (AC)11 | 2 | 137–150 | 60 | JN167560 |
HA-5 | F: GCGTGGATGTTCTTTCTT R: TCGAACCCTATGGATGTA | (GA)8 | 3 | 155–175 | 61 | JN167561 |
HA-6 | F: GAACAAGCCTGTCACTAA R: CACAAACCGATTTACGAT | (CT)18 | 2 | 130–178 | 60 | JN167562 |
HA-7 | F: CAGCAGTTAGAGCAGGAGGT R: TTCGGACATGAGTATGGGAT | (TG)8 | 3 | 208–215 | 59 | JN167563 |
HA-8 | F: CACTTCCACGAAGCTCTTAC R: GGAGATAAACAGAAAAGGGTA | (CTTCT)3 | 2 | 230–242 | 59 | JN167564 |
HA-9 | F: TATGGGTTTAGTGCCTGTAT R: TAGGTTGCTTGAATCTTTTC | (AC)9 | 2 | 322–341 | 59 | JN167565 |
HA-10 | F: CCCAAACCTCTATCATCT R: ATATCCCTTAGTTCTGCT | (GT)11 | 2 | 197–204 | 59 | JN167566 |
HA-11 | F: CACCAAATCCTGGAGAAGTA R: GGCAAACGAGAATAATCAAA | (AC)9 | 3 | 98–109 | 58 | JN167567 |
HA-12 | F: AAGGAGAAGCCAAGGTGAAA R: GACAAACCCACATACAGGAA | (GAA)5 | 2 | 119–132 | 60 | JN167568 |
HA-13 | F: ACTTTTATCGTATAGACCAG R: GAACACCTTTATTTCAGTGT | (CTT)15 | 2 | 110–118 | 59 | JN167569 |
HA-14 | F: GAAATGGCAAGGTTTTAGAT R: CTCAACTTTTGTGATGTGGC | (GAA)10 | 3 | 144–153 | 59 | JN167570 |
HA-15 | F: CAGCCACAATCCTCCTAACT R: GAAGGGTAACTTGTTTCACG | (TG)11 | 3 | 313–345 | 60 | JN167571 |
HA-16 | F: TTGAGATTTGACCTGGAA R: ACATTGGCGAAGATACAC | (CT)20 | 1 | 237 | 57 | JN167572 |
HA-17 | F: TATTTCCCTGTCCCTGTT R: GACCTTTTCGTCTTTTGG | (CT)14 | 1 | 74 | 54 | JN167573 |
HA-18 | F: CACCCAAGCATGATAAAA R: AGAATGAAAGAAAATGGC | (AC)14 | 1 | 164 | 58 | JN167574 |
HA-19 | F: ACCACCAGAAAGCAAACA R: GATGACTAATGGGAAAGAA | (CT)12 | 1 | 122 | 58 | JN167575 |
HA-20- | F: TCGTGATGGGAACAGATA R: TGAAATACTCATGGGAATG | (GA)13 | 1 | 141 | 57 | JN167576 |
HA-21 | F: AGAAAATCCCAATCTCAA R: CTAGCCAGAAACAACGAG | (CT)16 | 1 | 201 | 61 | JN167577 |
HA-22 | F: ACTGGTAACATCCCTGAC R: GAAACTGCTGGAAATCTA | (AC)9 | 1 | 107 | 60 | JN167578 |
HA-23 | F: AGCATCCGATCCTTATCT R: TATCAGCGACTCCTCCAC | (CT)16 | 1 | 158 | 59 | JN167579 |
HA-24 | F: AGTCATCGGAGAAATAGAG R: ATAACCAAGGAGGAAACA | (CAT)9 | 1 | 442 | 58 | JN167580 |
HA-25 | F: AAACTGCGAAATCCTCAT R: AGTAAACACTGCCTCCAT | (CT)16(CA)8 | 1 | 100 | 60 | JN167581 |
HA-26 | F: CCTCCGTGGTAACTCCTT R: TGATGAAATATGGCTTGG | (CA)11 | 1 | 137 | 59 | JN167582 |
HA-27 | F: TGAATTTCTTTTCTTCCTTTAC R: CAACTATCATCTTGTCGTGC | (TG)10 | 1 | 207 | 58 | JN167583 |
HA-28 | F: ATAGATGAACCAGGAAAT R: CTGAAGATAAAGAAAGCA | (AC)10 | 1 | 102 | 53 | JN167584 |
HA-29 | F: ATACGACAGATGCGGAAGTG R: TTAGTTACGGGAACCGAAGG | (GAA)3 | 1 | 196 | 60 | JN167585 |
HA-30 | F: TTGCTCACTTGAAAACATTA R: GAAAACGACACGATCACTCT | (AC)10 | 1 | 72 | 58 | JN167586 |
HA-31 | F: GGAAAGTGGCTGACTGGTAG R: CGACATCGGTGAGGTTGGTT | (AC)7 | 1 | 296 | 60 | JN167587 |
HA-32 | F: ACGGAAATGCTCAAACCCTC R: AAATGATTACCGCCGACAAC | (CTT)3 | 1 | 121 | 58 | JN167588 |
HA-33 | F: GCTCAGGTAAACCCATAA R: GCTCGTCGTACATACACTT | (CT)7 | 1 | 300 | 55 | JN167589 |
HA-34 | F: TACTGTCCAATGAATGCCTT R: AACCTGAACTATAAAGTAAACTGC | (GT)14 | 1 | 155 | 60 | JN167590 |
HA-35 | F: GTATGTTGCTATCCCCTAT R: CAAACCAAACAACACTAA | (TG)8 | 1 | 139 | 60 | JN167591 |
HA-36 | F: GAAAGGAATTGTACGTGGCA R: TATGGCTTGGGATTGGTTTT | (CA)8 | 1 | 264 | 60 | JN167592 |
HA-37 | F: TAAGATGGTATTGGAAGGG R: AGGGAGCATAAAAGTGGT | (GT)11 | 1 | 345 | 60 | JN167593 |
HA-38 | F: ATCATCGGCAGCGACTAG R: CAGCAAGGACATCAGGGT | (GA)13 | 1 | 203 | 59 | JN167594 |
HA-39 | F: TCCAAGATACTGCCATAC R: GGTTCTACAGGTACATGC | (AC)14 | 1 | 108 | 60 | JN167595 |
HA-40 | F: GAAGAGCGACAGAAAATG R: GGAAAATAAACAAGGGTAAA | (AC)14 | 1 | 103 | 59 | JN167596 |
Population 1 (N = 10) | Population 2 (N = 10) | Population 3 (N = 10) | Population 4 (N = 9) | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
Locus | NA | HO | HE | NA | HO | HE | NA | HO | HE | NA | HO | HE |
HA-1 | 2 | 0.51 | 0.70 | 2 | 0.51 | 0.80 | 2 | 0.48 | 0.70 | 2 | 0.47 | 0.67 |
HA-2 | 5 | 0.72 | 1.00 | 4 | 0.49 | 0.60 | 3 | 0.59 | 0.60 | 4 | 0.66 | 0.78 |
HA-3 | 2 | 0.19 | 0.20 | 2 | 0.48 | 0.10 | 2 | 0.47 | 0.40 | 2 | 0.52 | 0.00 |
HA-4 | 2 | 0.51 | 0.80 | 2 | 0.39 | 0.50 | 2 | 0.48 | 0.70 | 2 | 0.53 | 1.00 |
HA-5 | 3 | 0.62 | 0.70 | 3 | 0.57 | 0.40 | 3 | 0.54 | 0.70 | 3 | 0.46 | 0.56 |
HA-6 | 2 | 0.48 | 0.70 | 2 | 0.39 | 0.50 | 2 | 0.48 | 0.70 | 2 | 0.53 | 1.00 |
HA-7 | 3 | 0.47 | 0.50 | 2 | 0.44 | 0.60 | 3 | 0.56 | 0.90 | 2 | 0.37 | 0.44 |
HA-8 | 2 | 0.52 | 0.70 | 3 | 0.57 | 0.60 | 3 | 0.65 | 0.70 | 2 | 0.53 | 0.78 |
HA-9 | 2 | 0.44 | 0.60 | 2 | 0.52 | 0.70 | 2 | 0.44 | 0.60 | 2 | 0.50 | 0.78 |
HA-10 | 2 | 0.39 | 0.50 | 2 | 0.44 | 0.60 | 2 | 0.39 | 0.50 | 2 | 0.37 | 0.44 |
HA-11 | 3 | 0.51 | 0.70 | 2 | 0.51 | 0.60 | 3 | 0.62 | 0.70 | 3 | 0.69 | 0.78 |
HA-12 | 2 | 0.44 | 0.60 | 3 | 0.66 | 1.00 | 3 | 0.65 | 0.90 | 3 | 0.60 | 0.89 |
HA-13 | 2 | 0.39 | 0.30 | 3 | 0.57 | 0.60 | 3 | 0.42 | 0.30 | 3 | 0.65 | 0.56 |
HA-14 | 3 | 0.42 | 0.40 | 4 | 0.57 | 0.30 | 3 | 0.43 | 0.20 | 3 | 0.57 | 0.44 |
HA-15 | 3 | 0.53 | 0.70 | 3 | 0.61 | 0.70 | 3 | 0.68 | 1.00 | 3 | 0.68 | 0.89 |
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Zhang, L.; Sun, W.; Wang, Z.; Guan, K.; Yang, J. Isolation and Characterization of Microsatellite Loci for Hibiscus aridicola (Malvaceae), an Endangered Plant Endemic to the Dry-Hot Valleys of Jinsha River in Southwest China. Int. J. Mol. Sci. 2011, 12, 5698-5704. https://doi.org/10.3390/ijms12095698
Zhang L, Sun W, Wang Z, Guan K, Yang J. Isolation and Characterization of Microsatellite Loci for Hibiscus aridicola (Malvaceae), an Endangered Plant Endemic to the Dry-Hot Valleys of Jinsha River in Southwest China. International Journal of Molecular Sciences. 2011; 12(9):5698-5704. https://doi.org/10.3390/ijms12095698
Chicago/Turabian StyleZhang, Le, Weibang Sun, Zhonglang Wang, Kaiyun Guan, and Junbo Yang. 2011. "Isolation and Characterization of Microsatellite Loci for Hibiscus aridicola (Malvaceae), an Endangered Plant Endemic to the Dry-Hot Valleys of Jinsha River in Southwest China" International Journal of Molecular Sciences 12, no. 9: 5698-5704. https://doi.org/10.3390/ijms12095698