The Effects of H2S on the Activities of CYP2B6, CYP2D6, CYP3A4, CYP2C19 and CYP2C9 in Vivo in Rat
Abstract
:1. Introduction
2. Results and Discussion
2.1. Method Validation
2.2. Pharmacokinetic Study
3. Material and Methods
3.1. Chemicals and Reagents
3.2. Animals
3.3. Instrumentation and Conditions
3.4. Preparation of Standard Solutions
3.5. Pharmacokinetic Study
4. Conclusion
Acknowledgments
Conflicts of Interest
References
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Compound | Group | AUC (0–t) ng/mL h | AUC (0–∞) ng/mL h | MRT (0–t) h | MRT (0–∞) h | t1/2 h | Tmax h | CL L/h/kg | V L/kg | Cmax ng/mL |
---|---|---|---|---|---|---|---|---|---|---|
Bupropion | H2S | 393.0 ± 163.5 | 451.1 ± 176.3 | 1.8 ± 0.3 | 2.8 ± 1.3 | 2.3 ± 1.0 | 0.5 ± 0.1 | 21.7 ± 12.7 | 68.9 ± 40.3 | 308.4 ± 164.9 |
Normal | 532.7 ± 141.4 | 603.6 ± 123.0 | 2.1 ± 0.3 | 3.0 ± 1.0 | 1.9 ± 0.7 | 0.8 ± 0.4 | 13.7 ± 2.8 | 39.3 ± 17.9 | 279.2 ± 119.3 | |
Metoprolol | H2S | 627.7 ± 262.9 | 641.3 ± 265.9 | 1.5 ± 0.1 | 1.7 ± 0.1 | 1.1 ± 0.2 | 0.5 ± 0.1 | 14.3 ± 5.8 | 24.2 ± 12.1 | 447.1 ± 188.5 |
Normal | 693.8 ± 172.4 | 704.6 ± 168.7 | 1.5 ± 0.3 | 1.6 ± 0.3 | 1.0 ± 0.3 | 0.7 ± 0.2 | 12.0 ± 3.0 | 18.2 ± 8.2 | 463.3 ± 139.1 | |
Midazolam | H2S | 210.0 ± 95.5 * | 225.0 ± 100.7 * | 1.7 ± 0.3 | 2.2 ± 0.6 | 1.7 ± 0.3 | 0.4 ± 0.1 | 44.6 ± 25.8 * | 114.6 ± 92.6 | 152.9 ± 91.5 |
Normal | 509.2 ± 244.8 | 535.1 ± 239.4 | 1.6 ± 0.1 | 2.2 ± 0.7 | 1.7 ± 1.1 | 0.3 ± 0.1 | 18.6 ± 10.5 | 58.1 ± 73.1 | 330.9 ± 117.1 | |
Omeprazole | H2S | 446.7 ± 90.2 | 540.9 ± 90.5 | 1.2 ± 0.3 | 2.2 ± 0.7 | 1.9 ± 0.4 | 0.3 ± 0.2 | 15.2 ± 2.7 | 41.3 ± 11.8 | 536.7 ± 226.7 |
Normal | 427.4 ± 155.2 | 479.9 ± 165.4 | 1.1 ± 0.2 | 1.7 ± 0.4 | 1.7 ± 0.6 | 0.4 ± 0.2 | 18.1 ± 5.0 | 43.9 ± 21.5 | 491.0 ± 276.0 | |
Tolbutamide | H2S | 10,429.3 ± 2871.6 * | 11,099.1 ± 3218.4 * | 12.2 ± 2.7 | 14.8 ± 4.0 | 10.1 ± 2.7 | 2.9 ± 1.4 | 0.08 ± 0.02 * | 1.1 ± 0.3 | 640.4 ± 162.4 |
Normal | 6799.3 ± 769.0 | 7261.5 ± 916.4 | 12.7 ± 1.9 | 16.0 ± 5.8 | 10.6 ± 4.7 | 3.6 ± 3.2 | 0.11 ± 0.02 | 1.6 ± 0.6 | 484.7 ± 98.3 |
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Wang, X.; Han, A.; Wen, C.; Chen, M.; Chen, X.; Yang, X.; Ma, J.; Lin, G. The Effects of H2S on the Activities of CYP2B6, CYP2D6, CYP3A4, CYP2C19 and CYP2C9 in Vivo in Rat. Int. J. Mol. Sci. 2013, 14, 24055-24063. https://doi.org/10.3390/ijms141224055
Wang X, Han A, Wen C, Chen M, Chen X, Yang X, Ma J, Lin G. The Effects of H2S on the Activities of CYP2B6, CYP2D6, CYP3A4, CYP2C19 and CYP2C9 in Vivo in Rat. International Journal of Molecular Sciences. 2013; 14(12):24055-24063. https://doi.org/10.3390/ijms141224055
Chicago/Turabian StyleWang, Xianqin, Anyue Han, Congcong Wen, Mengchun Chen, Xinxin Chen, Xuezhi Yang, Jianshe Ma, and Guanyang Lin. 2013. "The Effects of H2S on the Activities of CYP2B6, CYP2D6, CYP3A4, CYP2C19 and CYP2C9 in Vivo in Rat" International Journal of Molecular Sciences 14, no. 12: 24055-24063. https://doi.org/10.3390/ijms141224055