Cardiac Ablation of Rheb1 Induces Impaired Heart Growth, Endoplasmic Reticulum-Associated Apoptosis and Heart Failure in Infant Mice
Abstract
:1. Introduction
2. Results and Discussion
2.1. Results
2.1.1. Development of Rheb1 cKO Mouse
2.1.2. Loss of Rheb1 Causes Heart Failure, Malignant Arrhythmias and Premature Death at Infant Stage
2.1.3. Cardiac Deletion of Rheb1 Results in Abnormal Heart Growth and Retarded Cardiomyocyte Size
2.1.4. Rheb1 Deletion Results in a Disrupted Expression Profile of Metabolic Genes and Increased Cardiomyocyte Apoptosis, but Does not Affect Autophagy
2.1.5. Knockout of Rheb1 Results in the Activation of Endoplasmic Reticulum (ER)-Associated Apoptosis Signaling Pathways
2.1.6. Knockout of Rheb1 Abolishes mTORC1 Signaling
2.1.7. Removal of Pten Prolongs Survival of Rheb1 cKO Mice
2.2. Discussion
3. Experimental Section
3.1. Animal Model
3.2. Echocardiography Assessment of Cardiac Function
3.3. Western Blot Analyses
3.4. Histology and Immunofluorescence Staining
3.5. TUNEL Assay and Transmission Electron Microscopy (TEM) Study
3.6. Quantitative Real-Time PCR for Metabolism Relative Genes and Fetal Genes
3.7. Mouse Cardiomyocyte Isolation
3.8. Statistical Analysis
4. Conclusions
Supplementary Information
ijms-14-24380-s001.pdfAcknowledgments
Conflicts of Interest
References
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Cao, Y.; Tao, L.; Shen, S.; Xiao, J.; Wu, H.; Li, B.; Wu, X.; Luo, W.; Xiao, Q.; Hu, X.; et al. Cardiac Ablation of Rheb1 Induces Impaired Heart Growth, Endoplasmic Reticulum-Associated Apoptosis and Heart Failure in Infant Mice. Int. J. Mol. Sci. 2013, 14, 24380-24398. https://doi.org/10.3390/ijms141224380
Cao Y, Tao L, Shen S, Xiao J, Wu H, Li B, Wu X, Luo W, Xiao Q, Hu X, et al. Cardiac Ablation of Rheb1 Induces Impaired Heart Growth, Endoplasmic Reticulum-Associated Apoptosis and Heart Failure in Infant Mice. International Journal of Molecular Sciences. 2013; 14(12):24380-24398. https://doi.org/10.3390/ijms141224380
Chicago/Turabian StyleCao, Yunshan, Lichan Tao, Shutong Shen, Junjie Xiao, Hang Wu, Beibei Li, Xiangqi Wu, Wen Luo, Qi Xiao, Xiaoshan Hu, and et al. 2013. "Cardiac Ablation of Rheb1 Induces Impaired Heart Growth, Endoplasmic Reticulum-Associated Apoptosis and Heart Failure in Infant Mice" International Journal of Molecular Sciences 14, no. 12: 24380-24398. https://doi.org/10.3390/ijms141224380