Borneol Depresses P-Glycoprotein Function by a NF-κB Signaling Mediated Mechanism in a Blood Brain Barrier in Vitro Model
Abstract
:1. Introduction
2. Results
2.1. Cell Characterization and Establishment of an in Vitro BBB Model
2.2. Borneol Down-Regulated P-gp Efflux Function
2.3. Effects of Borneol on mdr mRNA and P-gp Expression
2.4. Depressed P-gp Expression in BMECs by Borneol via a NF-κB Mediated Mechanism
3. Discussion
4. Experimental Section
4.1. Materials
4.2. Isolation and Culture of Rat Brain Microvascular Endothelial Cells
4.3. Isolation and Culture of Rat Astrocytes
4.4. The Establishment of an in Vitro BBB Model
4.5. Measurement of Transendothelial Electrical Resistance (TEER)
4.6. Measurement of γ-GT Activity in BMECs of the in Vitro BBB Model
4.7. The Effects of Borneol on Rho123 Accumulation in BMECs
4.8. The Effects of Borneol on P-gp Substrates Transport through the in Vitro BBB Model
4.9. Real-Time RT-PCR Analysis
4.10. Western Blot Analysis
4.11. Statistical Analysis
5. Conclusions
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Fan, X.; Chai, L.; Zhang, H.; Wang, Y.; Zhang, B.; Gao, X. Borneol Depresses P-Glycoprotein Function by a NF-κB Signaling Mediated Mechanism in a Blood Brain Barrier in Vitro Model. Int. J. Mol. Sci. 2015, 16, 27576-27588. https://doi.org/10.3390/ijms161126051
Fan X, Chai L, Zhang H, Wang Y, Zhang B, Gao X. Borneol Depresses P-Glycoprotein Function by a NF-κB Signaling Mediated Mechanism in a Blood Brain Barrier in Vitro Model. International Journal of Molecular Sciences. 2015; 16(11):27576-27588. https://doi.org/10.3390/ijms161126051
Chicago/Turabian StyleFan, Xiang, Lijuan Chai, Han Zhang, Yuefei Wang, Boli Zhang, and Xiumei Gao. 2015. "Borneol Depresses P-Glycoprotein Function by a NF-κB Signaling Mediated Mechanism in a Blood Brain Barrier in Vitro Model" International Journal of Molecular Sciences 16, no. 11: 27576-27588. https://doi.org/10.3390/ijms161126051