The Deubiquitinase USP17 Regulates the Stability and Nuclear Function of IL-33
Abstract
:1. Introduction
2. Results
2.1. IL-33 Can Be Modified by Protein Polyubiquitination
2.2. Ubiquitin-Specific Protease 17 (USP17) Associates with IL-33
2.3. USP17 Deubiquitinates IL-33
2.4. USP17 Stabilizes IL-33
2.5. IL-33 Upregulates IL-13 by Chromatin Binding to the IL13 Gene Locus
2.6. USP17 Downregulates the Chromatin Binding of IL-33 to the IL13 Gene Locus
3. Discussion
4. Materials and Methods
4.1. Cell Culture and Transfection
4.2. Antibodies and Reagents
4.3. Plasmid Constructs and Stable Cell Line Construction
4.4. Immunoprecipitation and Immune Blot
4.5. Ubiquitin Pull-down Assay
4.6. Quantitative PCR
- IL-33 forward: 5′-GAATCAGGTGACGGTGTTGA-3′;
- IL-33 reverse: 5′-TCCAGGATCAGTCTTGCATTC3′;
- β-actin forward: 5′-GGACTTCGAGCAAGAGATGG-3′;
- β-actin reverse: 5′-AGCACTGTGTTGGCGTACAG-3′.
4.7. Rapid Micro-Chromatin Immunoprecipitation Assay on Quantitative PCR
- Region1-F: 5′-CGACACTGGATTTTCCACAA-3′;
- Region1-R: 5′-GCCAACAGGAGAGGATTGAG-3′;
- Region2-F: 5′-GGGTTGTCACAGGCAAAACT-3′;
- Region2-R: 5′-TTGTGGAAAATCCAGTGTCG-3′;
- Region3-F: 5′-ACTCCCCAAATTCCCATAGC-3′;
- Region3-R: 5′-CCAAGATCTGGGTGGGTTTA-3′;
- Region4-F: 5′-GTGAGACCCCATCTCCAAAA-3′;
- Region4-R: 5′-GGCTTGCCTAATTCCTCCTT-3′;
- Region5-F: 5′-GAGGGAAGAGCAGGAAAAGG-3′;
- Region5-R: 5′-GCAGCCTTAGTCCAGGTCAG-3′;
- Region6-F: 5′-GCTTCGAGTGTGGACAGAGA-3′;
- Region6-R: 5′-TTCTCCCCTACCCACTTCCT-3′;
- Region7-F: 5′-GATAAGGGGCGTTGACTCAC-3′;
- Region7-R: 5′-CGTGTGACCCCTCTACGG-3′.
Acknowledgments
Author Contributions
Conflicts of Interest
Abbreviations
References
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Ni, Y.; Tao, L.; Chen, C.; Song, H.; Li, Z.; Gao, Y.; Nie, J.; Piccioni, M.; Shi, G.; Li, B. The Deubiquitinase USP17 Regulates the Stability and Nuclear Function of IL-33. Int. J. Mol. Sci. 2015, 16, 27956-27966. https://doi.org/10.3390/ijms161126063
Ni Y, Tao L, Chen C, Song H, Li Z, Gao Y, Nie J, Piccioni M, Shi G, Li B. The Deubiquitinase USP17 Regulates the Stability and Nuclear Function of IL-33. International Journal of Molecular Sciences. 2015; 16(11):27956-27966. https://doi.org/10.3390/ijms161126063
Chicago/Turabian StyleNi, Yingmeng, Lianqin Tao, Chen Chen, Huihui Song, Zhiyuan Li, Yayi Gao, Jia Nie, Miranda Piccioni, Guochao Shi, and Bin Li. 2015. "The Deubiquitinase USP17 Regulates the Stability and Nuclear Function of IL-33" International Journal of Molecular Sciences 16, no. 11: 27956-27966. https://doi.org/10.3390/ijms161126063