Probing Peptide and Protein Insertion in a Biomimetic S-Layer Supported Lipid Membrane Platform
Abstract
:1. Introduction
2. Results and Discussion
2.1. Assembly of S-Layer Supported Lipid Membranes
2.2. Electrical Characterization of S-Layer Supported Lipid Membranes
2.3. Incorporation of Gramicidin
2.4. Reconstitution of α-Hemolysin
3. Experimental Section
3.1. Isolation of S-Layer Proteins
3.2. EDC Activation
3.3. Vesicle Preparation
3.5. Quartz Crystal Microbalance with Dissipation Measurement (QCM-D)
3.6. Electrochemical Impedance Spectroscopy (EIS)
4. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Damiati, S.; Schrems, A.; Sinner, E.-K.; Sleytr, U.B.; Schuster, B. Probing Peptide and Protein Insertion in a Biomimetic S-Layer Supported Lipid Membrane Platform. Int. J. Mol. Sci. 2015, 16, 2824-2838. https://doi.org/10.3390/ijms16022824
Damiati S, Schrems A, Sinner E-K, Sleytr UB, Schuster B. Probing Peptide and Protein Insertion in a Biomimetic S-Layer Supported Lipid Membrane Platform. International Journal of Molecular Sciences. 2015; 16(2):2824-2838. https://doi.org/10.3390/ijms16022824
Chicago/Turabian StyleDamiati, Samar, Angelika Schrems, Eva-Kathrin Sinner, Uwe B. Sleytr, and Bernhard Schuster. 2015. "Probing Peptide and Protein Insertion in a Biomimetic S-Layer Supported Lipid Membrane Platform" International Journal of Molecular Sciences 16, no. 2: 2824-2838. https://doi.org/10.3390/ijms16022824