Chromium-Induced Ultrastructural Changes and Oxidative Stress in Roots of Arabidopsis thaliana
Abstract
:1. Introduction
2. Results
2.1. Morphological Effects, Reactive Oxygen Species (ROS) Disturbance and Cell Mortality
2.2. Ultrastructural Malformations
3. Discussion
3.1. Electron Dense Deposits, Cell Wall and Cell Membranes
3.2. Ultrastructural Effects and Oxidative Stress
4. Experimental Section
4.1. Plant Material and Preliminary Experiments
4.2. Detection of Dead Cells with Evans Blue and Trypan Blue
4.3. Callose Detection with Aniline Blue
4.4. Detection of Hydrogen Peroxide Production with DCF-DA
4.5. Detection of Peroxidase Activity with Pyrogallol
4.6. Light and Transmission Electron Microscopy
5. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Eleftheriou, E.P.; Adamakis, I.-D.S.; Panteris, E.; Fatsiou, M. Chromium-Induced Ultrastructural Changes and Oxidative Stress in Roots of Arabidopsis thaliana. Int. J. Mol. Sci. 2015, 16, 15852-15871. https://doi.org/10.3390/ijms160715852
Eleftheriou EP, Adamakis I-DS, Panteris E, Fatsiou M. Chromium-Induced Ultrastructural Changes and Oxidative Stress in Roots of Arabidopsis thaliana. International Journal of Molecular Sciences. 2015; 16(7):15852-15871. https://doi.org/10.3390/ijms160715852
Chicago/Turabian StyleEleftheriou, Eleftherios P., Ioannis-Dimosthenis S. Adamakis, Emmanuel Panteris, and Maria Fatsiou. 2015. "Chromium-Induced Ultrastructural Changes and Oxidative Stress in Roots of Arabidopsis thaliana" International Journal of Molecular Sciences 16, no. 7: 15852-15871. https://doi.org/10.3390/ijms160715852