Investigation of Enantioselective Membrane Permeability of α-Lipoic Acid in Caco-2 and MDCKII Cell
Abstract
:1. Introduction
2. Results and Discussion
Side | Time (min) | Concentrations (µg/mL) | |||||
---|---|---|---|---|---|---|---|
Low Group | Middle Group | High Group | |||||
RLA | SLA | RLA | SLA | RLA | SLA | ||
basal | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
15 | 0.06 ± 0.01 | 0.07 ± 0.01 | 0.36 ± 0.08 | 0.38 ± 0.10 | 1.61 ± 0.27 | 1.64 ± 0.28 | |
30 | 0.14 ± 0.01 | 0.15 ± 0.01 | 0.70 ± 0.09 | 0.73 ± 0.08 | 3.54 ± 0.39 | 3.66 ± 0.36 | |
60 | 0.27 ± 0.1 | 0.28 ± 0.01 | 1.28 ± 0.19 | 1.32 ± 0.20 | 7.09 ± 0.18 | 7.34 ± 0.14 | |
120 | 0.43 ± 0.02 * | 0.46 ± 0.02 | 2.35 ± 0.12 * | 2.47 ± 0.14 | 11.80 ± 0.38 | 12.19 ± 0.30 | |
apical | 120 | 1.94 ± 0.04 | 2.02 ± 0.05 | 10.64 ± 0.12 | 11.05 ± 0.28 | 65.01 ± 4.04 | 67.56 ± 3.47 |
Cell Types | Papp (×10−6 cm/s) | |||||
---|---|---|---|---|---|---|
Low Group | Middle Group | High Group | ||||
RLA | SLA | RLA | SLA | RLA | SLA | |
Caco-2 | 14.3 ± 2.2 | 15.4 ± 2.2 | 15.9 ± 3.7 | 16.7 ± 4.3 | 14.4 ± 2.4 | 14.6 ± 2.5 |
Side | Time (min) | Concentrations (µg/mL) | |||||
---|---|---|---|---|---|---|---|
Low Group | Middle Group | High Group | |||||
RLA | SLA | RLA | SLA | RLA | SLA | ||
basal | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
15 | 0.90 ± 0.02 | 0.90 ± 0.03 | 4.90 ± 0.09 | 4.89 ± 0.10 | 23.00 ± 1.61 | 23.50 ± 1.65 | |
30 | 1.51 ± 0.03 | 1.52 ± 0.02 | 8.48 ± 0.57 | 8.57 ± 0.64 | 37.84 ± 2.21 | 38.79 ± 2.19 | |
60 | 2.33 ± 0.11 | 2.40 ± 0.12 | 12.82 ± 0.63 | 12.85 ± 0.68 | 58.44 ± 1.68 | 59.76 ± 1.73 | |
120 | 3.11 ± 0.17 | 3.04 ± 0.16 | 17.83 ± 1.20 | 17.74 ± 1.44 | 81.65 ± 2.68 | 83.69 ± 3.36 | |
apical | 120 | 0.86 ± 0.01 | 0.84 ± 0.02 | 6.02 ± 0.77 | 5.74 ± 0.62 | 59.23 ± 1.36 | 60.17 ± 1.46 |
Cell Types | Papp (×10−6 cm/s) | |||||
---|---|---|---|---|---|---|
Low Group | Middle Group | High Group | ||||
RLA | SLA | RLA | SLA | RLA | SLA | |
MDCK II | 185.4 ± 5.5 | 186.2 ± 4.6 | 197.3 ± 2.6 | 197.7 ± 2.0 | 186.3 ± 12.7 | 189.8 ± 13.9 |
3. Experimental Section
3.1. Chemical and Reagents
3.2. Cell Culture
3.2.1. Caco-2
3.2.2. MDCK II
3.3. Transport Experiments
3.3.1. Caco-2
3.3.2. MDCK II
3.4. Determination of LA Concentration by LC-MS/MS
3.5. Data Analysis
4. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Uchida, R.; Okamoto, H.; Ikuta, N.; Terao, K.; Hirota, T. Investigation of Enantioselective Membrane Permeability of α-Lipoic Acid in Caco-2 and MDCKII Cell. Int. J. Mol. Sci. 2016, 17, 155. https://doi.org/10.3390/ijms17020155
Uchida R, Okamoto H, Ikuta N, Terao K, Hirota T. Investigation of Enantioselective Membrane Permeability of α-Lipoic Acid in Caco-2 and MDCKII Cell. International Journal of Molecular Sciences. 2016; 17(2):155. https://doi.org/10.3390/ijms17020155
Chicago/Turabian StyleUchida, Ryota, Hinako Okamoto, Naoko Ikuta, Keiji Terao, and Takashi Hirota. 2016. "Investigation of Enantioselective Membrane Permeability of α-Lipoic Acid in Caco-2 and MDCKII Cell" International Journal of Molecular Sciences 17, no. 2: 155. https://doi.org/10.3390/ijms17020155