Knockdown of AMPKα2 Promotes Pulmonary Arterial Smooth Muscle Cells Proliferation via mTOR/Skp2/p27Kip1 Signaling Pathway
Abstract
:1. Introduction
2. Results
2.1. Knockdown of Adenosine Monophosphate-Activated Protein Kinase α2 (AMPKα2) Promotes Pulmonary Arterial Smooth Muscle Cells (PASMCs) Proliferation
2.2. Knockdown of AMPKα2 Increases the Phosphorylation of Mammalian Target of Rapamycin (mTOR), Upregulates S-Phase Kinase-Associated Protein 2 (Skp2) and Downregulates p27Kip1
2.3. mTOR Mediates AMPKα2 Knockdown-Induced Skp2 Upregulation, p27Kip1 Downregulation and PASMCs Proliferation
2.4. Skp2 Mediates AMPKα2 Knockdown-Induced p27Kip1 Reduction and PASMCs Proliferation
3. Discussion
4. Materials and Methods
4.1. Cell Culture
4.2. siRNA Transfection
4.3. BrdU Incorporation Assay
4.4. Immunoblotting
4.5. Statistics
Acknowledgments
Author Contributions
Conflicts of Interest
Abbreviations
AICAR | 5-aminoimidazole-4-carboxamide ribonucleoside |
AMPK | adenosine monophosphate-activated protein kinase |
CDKs | cyclin-dependent kinases |
mTOR | mammalian target of rapamycin |
PAH | pulmonary arterial hypertension |
PASMCs | pulmonary arterial smooth muscle cells |
RNAi | RNA interference |
Skp2 | S-phase kinase-associated protein 2 |
siRNA | small interfering RNA |
TSC | tuberous sclerosis complex |
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Ke, R.; Liu, L.; Zhu, Y.; Li, S.; Xie, X.; Li, F.; Song, Y.; Yang, L.; Gao, L.; Li, M. Knockdown of AMPKα2 Promotes Pulmonary Arterial Smooth Muscle Cells Proliferation via mTOR/Skp2/p27Kip1 Signaling Pathway. Int. J. Mol. Sci. 2016, 17, 844. https://doi.org/10.3390/ijms17060844
Ke R, Liu L, Zhu Y, Li S, Xie X, Li F, Song Y, Yang L, Gao L, Li M. Knockdown of AMPKα2 Promotes Pulmonary Arterial Smooth Muscle Cells Proliferation via mTOR/Skp2/p27Kip1 Signaling Pathway. International Journal of Molecular Sciences. 2016; 17(6):844. https://doi.org/10.3390/ijms17060844
Chicago/Turabian StyleKe, Rui, Lu Liu, Yanting Zhu, Shaojun Li, Xinming Xie, Fangwei Li, Yang Song, Lan Yang, Li Gao, and Manxiang Li. 2016. "Knockdown of AMPKα2 Promotes Pulmonary Arterial Smooth Muscle Cells Proliferation via mTOR/Skp2/p27Kip1 Signaling Pathway" International Journal of Molecular Sciences 17, no. 6: 844. https://doi.org/10.3390/ijms17060844