Antioxidant and Cytoprotective Activities of Fucus spiralis Seaweed on a Human Cell in Vitro Model
Abstract
:1. Introduction
2. Results
2.1. Antioxidant Activity
2.2. Protective Effect of Seaweed Fractions on MCF-7 Cells Exposed to H2O2
2.3. Study of the Cellular Mechanisms Involved in the Cytotoxicity Induced by H2O2 on MCF-7 Cells in the Presence or Absence of Fucus spiralis Fractions
2.3.1. Real-Time Quantification of H2O2 Production
2.3.2. Mitochondrial Membrane Potential (ΔΨm)
2.3.3. Caspase-9 Activity
3. Discussion
4. Materials and Methods
4.1. Chemicals and Reagents
4.2. Collection, Preparation and Extraction of Fucus spiralis
4.3. Fractionation of Fucus spiralis Methanolic Extract by Vacuum Liquid Chromatography (VLC)
4.4. Analysis of Total Phenolic Content (TPC)
4.5. Evaluation of Antioxidant Activities
4.5.1. DPPH (1,1-Diphenyl-2-picrylhydrazyl) Radical Scavenging Activity
4.5.2. Oxygen Radical Absorbance Capacity (ORAC)
4.5.3. Hydroxyl Radical Scavenging Activity (•OH)
4.6. In Vitro Assay of Oxidative Stress Prevention
4.6.1. Cell Maintenance Culture Conditions
4.6.2. Fucus spiralis Fractions Cytotoxicity Evaluation
4.6.3. Evaluation of the Protective Effect of Seaweed Fractions in an Oxidative Stress Condition Induced by H2O2 on MCF-7 Cells
4.6.4. Real-Time Quantification of H2O2 Production
4.6.5. Mitochondrial Membrane Potential (ΔΨm)
4.6.6. Caspase-9 Activity
4.7. Statistical Analysis
5. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Fractions | TPC a | DPPH b | •OH c | ORAC d |
---|---|---|---|---|
1 | 8.00 ± 1.10 | 182.90 (124.30–269.00) | 7.90 (5.60–11.60) | 2,988.00 ± 107.77 |
2 | 33.00 ± 18.00 | 44.40 (38.30–51.47) | 11.52 (8.03–16.52) | 5,411.00 ± 91.54 |
3 | 379.00 ± 34.0 | 15.58 (13.31–18.22) | 9.73 (6.51–14.55) | 6,877.00 ± 92.56 |
4 | 419.00 ± 3.00 | 13.94 (11.13–17.46) | 10.86 (5.90–19.95) | 34,893.68 ± 945.20 |
5 | 285.00 ± 12.00 | 9.74 (8.14–11.66) | 58.61 (41.32–83.14) | 30,691.00 ± 1172.90 |
BHT | - | 40.55 (25.74–63.87) | >1000 | 320.49 ± 32.31 |
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Pinteus, S.; Silva, J.; Alves, C.; Horta, A.; Thomas, O.P.; Pedrosa, R. Antioxidant and Cytoprotective Activities of Fucus spiralis Seaweed on a Human Cell in Vitro Model. Int. J. Mol. Sci. 2017, 18, 292. https://doi.org/10.3390/ijms18020292
Pinteus S, Silva J, Alves C, Horta A, Thomas OP, Pedrosa R. Antioxidant and Cytoprotective Activities of Fucus spiralis Seaweed on a Human Cell in Vitro Model. International Journal of Molecular Sciences. 2017; 18(2):292. https://doi.org/10.3390/ijms18020292
Chicago/Turabian StylePinteus, Susete, Joana Silva, Celso Alves, André Horta, Olivier P. Thomas, and Rui Pedrosa. 2017. "Antioxidant and Cytoprotective Activities of Fucus spiralis Seaweed on a Human Cell in Vitro Model" International Journal of Molecular Sciences 18, no. 2: 292. https://doi.org/10.3390/ijms18020292