Caloric Restriction Protects against Lactacystin-Induced Degeneration of Dopamine Neurons Independent of the Ghrelin Receptor
Abstract
:1. Introduction
2. Results
2.1. Deletion of the Ghrelin Receptor Does Not Induce DA-Ergic Degeneration in the SNc of Adult and Aged Mice
2.2. No Effect of Ghrelin Receptor Ablation on Metabolic Parameters during CR
2.3. Caloric Restriction Inhibits Nigral Cell Loss Following LAC Administration
2.4. Caloric Restriction Limits Striatal DA Loss in LAC-Injected Mice
3. Discussion
4. Material and Methods
4.1. Animals
4.2. Possible Neurodegeneration by Ghrelin Receptor Ablation
4.3. Caloric Restriction
4.4. Nigral LAC Lesioning
4.5. ELISA for Determination of Acetylated Ghrelin
4.6. Liquid Chromatographic Determination of Total DA Content in the Striatum
4.7. Immunohistochemistry for Detection of Tyrosine Hydroxylase (TH)-Containing Neurons
4.8. Statistical Analysis
5. Conclusions
Acknowledgments
Author Contributions
Conflicts of interest
References
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Coppens, J.; Bentea, E.; Bayliss, J.A.; Demuyser, T.; Walrave, L.; Albertini, G.; Van Liefferinge, J.; Deneyer, L.; Aourz, N.; Van Eeckhaut, A.; et al. Caloric Restriction Protects against Lactacystin-Induced Degeneration of Dopamine Neurons Independent of the Ghrelin Receptor. Int. J. Mol. Sci. 2017, 18, 558. https://doi.org/10.3390/ijms18030558
Coppens J, Bentea E, Bayliss JA, Demuyser T, Walrave L, Albertini G, Van Liefferinge J, Deneyer L, Aourz N, Van Eeckhaut A, et al. Caloric Restriction Protects against Lactacystin-Induced Degeneration of Dopamine Neurons Independent of the Ghrelin Receptor. International Journal of Molecular Sciences. 2017; 18(3):558. https://doi.org/10.3390/ijms18030558
Chicago/Turabian StyleCoppens, Jessica, Eduard Bentea, Jacqueline A. Bayliss, Thomas Demuyser, Laura Walrave, Giulia Albertini, Joeri Van Liefferinge, Lauren Deneyer, Najat Aourz, Ann Van Eeckhaut, and et al. 2017. "Caloric Restriction Protects against Lactacystin-Induced Degeneration of Dopamine Neurons Independent of the Ghrelin Receptor" International Journal of Molecular Sciences 18, no. 3: 558. https://doi.org/10.3390/ijms18030558