miR-30a as Potential Therapeutics by Targeting TET1 through Regulation of Drp-1 Promoter Hydroxymethylation in Idiopathic Pulmonary Fibrosis
Abstract
:1. Introduction
2. Results
2.1. miR-30a Expression in Patients and Analysis of the Possible Target Gene
2.2. Confirmation of Ten–Eleven Translocation 1(TET1) as a Target Gene of miR-30a
2.3. TET1 Interference Inhibited Dynamin-Related Protein1 (Drp-1) Promoter Hydroxymethylation
2.4. miR-30a as a Potential Therapeutic Target for Idiopathic Pulmonary Fibrosis (IPF)
3. Discussion
4. Materials and Methods
4.1. IPF Patients
4.2. Human Fetal Lung Fibroblast MRC-5 Cell Culture
4.3. Cell Transfection
4.4. Quantitative Real-Time Polymerase Chain Reaction (qRT-PCR)
4.5. Detection of Drp-1 Promoter Hydroxymethylation
4.6. Animal Model and Ethics Statement
4.7. Dual-Luciferase Assays
4.8. Hematoxylin and Eosin (H&E)and Masson’s Trichrome Staining
4.9. Western Blot Analysis
4.10. Statistical Analysis
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Characteristic | Normal | IPF | p Value |
---|---|---|---|
Number | 46 | 46 | / |
Age (years) | 68.29 | 68.17 | / |
Gender (Male/female) | 23/23 | 23/23 | / |
FVC (% of predicted) | 87.28 | 47.81 | <0.01 |
TLC (% of predicted) | 88.15 | 52.17 | <0.01 |
DLCO (% of predicted) | 89.17 | 63.75 | <0.01 |
PaO2 (mmHg) | 93.71 | 76.21 | <0.01 |
PaCO2 (mmHg) | 38.56 | 37.11 | / |
Smoking History (n) | 4 | 15 | <0.05 |
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Zhang, S.; Liu, H.; Liu, Y.; Zhang, J.; Li, H.; Liu, W.; Cao, G.; Xv, P.; Zhang, J.; Lv, C.; et al. miR-30a as Potential Therapeutics by Targeting TET1 through Regulation of Drp-1 Promoter Hydroxymethylation in Idiopathic Pulmonary Fibrosis. Int. J. Mol. Sci. 2017, 18, 633. https://doi.org/10.3390/ijms18030633
Zhang S, Liu H, Liu Y, Zhang J, Li H, Liu W, Cao G, Xv P, Zhang J, Lv C, et al. miR-30a as Potential Therapeutics by Targeting TET1 through Regulation of Drp-1 Promoter Hydroxymethylation in Idiopathic Pulmonary Fibrosis. International Journal of Molecular Sciences. 2017; 18(3):633. https://doi.org/10.3390/ijms18030633
Chicago/Turabian StyleZhang, Songzi, Huizhu Liu, Yuxia Liu, Jie Zhang, Hongbo Li, Weili Liu, Guohong Cao, Pan Xv, Jinjin Zhang, Changjun Lv, and et al. 2017. "miR-30a as Potential Therapeutics by Targeting TET1 through Regulation of Drp-1 Promoter Hydroxymethylation in Idiopathic Pulmonary Fibrosis" International Journal of Molecular Sciences 18, no. 3: 633. https://doi.org/10.3390/ijms18030633