Overexpression of S-Adenosyl-l-Methionine Synthetase 2 from Sugar Beet M14 Increased Arabidopsis Tolerance to Salt and Oxidative Stress
Abstract
:1. Introduction
2. Results
2.1. Identification of Differentially Expressed M14 Genes under Salt Stress Using SSH
2.2. Cloning of a BvM14-SAMS2 Gene and Sequence Analysis
2.3. Analysis of BvM14-SAMS2 Response to Salt Stress
2.4. Overexpression of BvM14-SAMS2 Confers Enhanced Salt and H2O2 Tolerance in Arabidopsis
2.5. Overexpression of BvM14-SAMS2 Increased Antioxidative Activities in Arabidopsis
2.6. Overexpression of BvM14-SAMS2 Greatly Influenced Polyamine Metabolism
3. Discussion
4. Materials and Methods
4.1. Plant Materials and Growth Conditions
4.2. Construction of a Subtractive cDNA Library
4.3. Molecular Cloning of BvM14-SAMS2 Gene and Sequence Analysis
4.4. Real-Time Quantitative PCR
4.5. Isolation of T-DNA Insertion Mutants in AtSAMS3
4.6. Constitutive Expression of BvM14-SAMS2 in Arabidopsis and Stress Tolerance Analysis
4.7. Determination of Antioxidant Enzyme Activities, Lipid Peroxidation, H2O2 Concentration, SAM Concentration and Polyamine Metabolism
4.8. Statistical Analysis
5. Conclusions
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Unigene Number | Length (bp) | Annotation | Score | E-Value |
---|---|---|---|---|
L1 | 354 | Glutathione S-transferase | 159 | 6 × 10−33 |
L2 | 231 | Late embryogenesis abundant protein | 73 | 1 × 10−5 |
L3 | 174 | Chloroplastic chlorophyll a-b binding 8 | 154 | 2 × 10−27 |
L4 | 150 | ATP-binding cassette transporter C family member 14 | 70 | 3 × 10−11 |
L5 | 392 | Ca2+ transporting ATPase | 260 | 4 × 10−78 |
L6 | 237 | MYB (v-myb avian myeloblastosis viral oncogene homolog) transcription factor | 52 | 2 × 10−7 |
L7 | 359 | Salt-induced hydrophilic protein | 70.3 | 2 × 10−9 |
L8 | 467 | Aldehyde dehydrogenase family 7 A1 | 119 | 1 × 10−32 |
L9 | 233 | Cysteine proteinase inhibitor | 134 | 1 × 10−8 |
L10 | 221 | Aldehyde dehydrogenase | 123 | 3 × 10−13 |
L11 | 274 | S-adenosylmethionine decarboxylase | 155 | 1 × 10−36 |
L12 | 148 | Pyruvate kinase family protein | 89 | 4 × 10−18 |
L13 | 274 | Cysteine protease | 63 | 1 × 10−7 |
L14 | 297 | Short-chain dehydrogenase | 73 | 7 × 10−11 |
L15 | 246 | Carboxyl-terminal-processing protease | 132 | 1 × 10−30 |
L16 | 301 | MYB transcription factor | 94 | 2 × 10−7 |
L17 | 144 | Short-chain dehydrogenases/reductases family 7C | 198 | 5 × 10−8 |
L18 | 326 | Vacuole ATPase subunit A | 144 | 3 × 10−66 |
L19 | 528 | Putative mitochondrial carrier protein | 163 | 5 × 10−77 |
L20 | 416 | Nitrite reductase | 254 | 4 × 10−66 |
L21 | 512 | Lipid transfer protein | 184 | 4 × 10−45 |
L22 | 314 | S-adenosylmethionine synthase 2 | 239 | 1 × 10−61 |
L23 | 763 | Phosphoglycerate kinase | 407 | 8 × 10−112 |
L24 | 236 | Cellulose synthase-like protein E6 | 71.2 | 5 × 10−11 |
L25 | 450 | High-mobility group B6 transcription factor | 69 | 3 × 10−2 |
L26 | 544 | Heat- and acid-stable phosphoprotein | 95 | 3 × 10−16 |
L27 | 236 | Thioredoxin domain 2 | 68 | 6 × 10−31 |
L28 | 437 | Hypothetical protein | 109 | 5 × 10−4 |
L29 | 197 | DNA replication licensing factor minichromosome maintenance 4 (MCM4) | 88 | 2 × 10−2 |
R1 | 245 | Thioredoxin domain 1 | 96 | 3 × 10−13 |
R2 | 364 | S-adenosylmethionine synthase 2 | 194 | 1 × 10−22 |
R3 | 208 | WD (Trp-Asp) repeat phosphoinositide-interacting | 174 | 6 × 10−30 |
R4 | 447 | AAA-type ATPase family protein | 143 | 4 × 10−12 |
R5 | 182 | Putative senescence-associated protein | 121 | 6 × 10−2 |
R6 | 394 | S-adenosylmethionine synthase 2 | 74 | 3 × 10−7 |
R7 | 113 | 60S ribosomal protein L19-3 | 863 | 4 × 10−14 |
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Ma, C.; Wang, Y.; Gu, D.; Nan, J.; Chen, S.; Li, H. Overexpression of S-Adenosyl-l-Methionine Synthetase 2 from Sugar Beet M14 Increased Arabidopsis Tolerance to Salt and Oxidative Stress. Int. J. Mol. Sci. 2017, 18, 847. https://doi.org/10.3390/ijms18040847
Ma C, Wang Y, Gu D, Nan J, Chen S, Li H. Overexpression of S-Adenosyl-l-Methionine Synthetase 2 from Sugar Beet M14 Increased Arabidopsis Tolerance to Salt and Oxidative Stress. International Journal of Molecular Sciences. 2017; 18(4):847. https://doi.org/10.3390/ijms18040847
Chicago/Turabian StyleMa, Chunquan, Yuguang Wang, Dan Gu, Jingdong Nan, Sixue Chen, and Haiying Li. 2017. "Overexpression of S-Adenosyl-l-Methionine Synthetase 2 from Sugar Beet M14 Increased Arabidopsis Tolerance to Salt and Oxidative Stress" International Journal of Molecular Sciences 18, no. 4: 847. https://doi.org/10.3390/ijms18040847