Combining Low Temperature Fluorescence DNA-Hybridization, Immunostaining, and Super-Resolution Localization Microscopy for Nano-Structure Analysis of ALU Elements and Their Influence on Chromatin Structure
Abstract
:1. Introduction
2. Results
2.1. Localization Microscopy after Immunohistochemistry Labelling
2.2. Probe Design for ALU-Combinatorial Oligonucleotide (COMBO)-Fluorescence In Situ Hybridization (FISH)-Staining
2.3. Two-Color Localization Microscopy and ALU Clustering
2.4. Biological Dosimetry by ALU Point Counting
3. Discussion
4. Materials and Methods
4.1. Staining Materials and Concentrations (Immunostaining and COMBO-FISH)
4.2. Handling Steps
4.3. Microscopy Setup
5. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Krufczik, M.; Sievers, A.; Hausmann, A.; Lee, J.-H.; Hildenbrand, G.; Schaufler, W.; Hausmann, M. Combining Low Temperature Fluorescence DNA-Hybridization, Immunostaining, and Super-Resolution Localization Microscopy for Nano-Structure Analysis of ALU Elements and Their Influence on Chromatin Structure. Int. J. Mol. Sci. 2017, 18, 1005. https://doi.org/10.3390/ijms18051005
Krufczik M, Sievers A, Hausmann A, Lee J-H, Hildenbrand G, Schaufler W, Hausmann M. Combining Low Temperature Fluorescence DNA-Hybridization, Immunostaining, and Super-Resolution Localization Microscopy for Nano-Structure Analysis of ALU Elements and Their Influence on Chromatin Structure. International Journal of Molecular Sciences. 2017; 18(5):1005. https://doi.org/10.3390/ijms18051005
Chicago/Turabian StyleKrufczik, Matthias, Aaron Sievers, Annkathrin Hausmann, Jin-Ho Lee, Georg Hildenbrand, Wladimir Schaufler, and Michael Hausmann. 2017. "Combining Low Temperature Fluorescence DNA-Hybridization, Immunostaining, and Super-Resolution Localization Microscopy for Nano-Structure Analysis of ALU Elements and Their Influence on Chromatin Structure" International Journal of Molecular Sciences 18, no. 5: 1005. https://doi.org/10.3390/ijms18051005