Novel Structurally Related Flavones Augment Cell Death Induced by rhsTRAIL
Abstract
:1. Introduction
2. Results
2.1. Cytotoxic and Apoptotic Effects of Flavones in Colon Cancer Cells
2.2. Cytotoxic and Apoptotic Effects of TRAIL in Combination with Flavones in Colon Cancer Cells
3. Discussion
4. Materials and Methods
4.1. Chemistry
General
4.2. General Procedure for the Esterification of 5-HF, 6-HF and 7-HF
4.3. Biological Methods
4.3.1. Reagents
4.3.2. Cell Culture
4.3.3. Detection of Cell Death Using the MTT Colorimetric Assay
4.3.4. Lactate Dehydrogenase Release Assay
4.3.5. Determination of Apoptosis by Flow Cytometry with Annexin V-FITC Staining
4.4. Statistical Analysis
5. Conclusions
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
Abbreviations
TRAIL | tumor necrosis factor-related apoptosis-inducing |
V-FITC | annexin fluorescence staining ligand |
TNF | tumor necrosis factor |
LDH | lactate dehydrogenase assay |
MTT | 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide |
DMSO | dimethylsulfoxide |
DMF | dimethylformamide |
PBS | phosphate-buffered saline |
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Bronikowska, J.; Szliszka, E.; Kostrzewa-Susłow, E.; Jaworska, D.; Czuba, Z.P.; Bednarski, P.; Król, W. Novel Structurally Related Flavones Augment Cell Death Induced by rhsTRAIL. Int. J. Mol. Sci. 2017, 18, 1211. https://doi.org/10.3390/ijms18061211
Bronikowska J, Szliszka E, Kostrzewa-Susłow E, Jaworska D, Czuba ZP, Bednarski P, Król W. Novel Structurally Related Flavones Augment Cell Death Induced by rhsTRAIL. International Journal of Molecular Sciences. 2017; 18(6):1211. https://doi.org/10.3390/ijms18061211
Chicago/Turabian StyleBronikowska, Joanna, Ewelina Szliszka, Edyta Kostrzewa-Susłow, Dagmara Jaworska, Zenon P. Czuba, Piotr Bednarski, and Wojciech Król. 2017. "Novel Structurally Related Flavones Augment Cell Death Induced by rhsTRAIL" International Journal of Molecular Sciences 18, no. 6: 1211. https://doi.org/10.3390/ijms18061211