CD9 and CD81 Interactions and Their Structural Modelling in Sperm Prior to Fertilization
Abstract
:1. Introduction
2. Results
2.1. Mutual Position of CD9 and CD81 on Mouse Sperm Head
2.2. Mutual Localization of CD9 and CD81 in Human Sperm Head
2.3. Immunoprecipitation of CD9 and CD81 Complex from Spermatozoa
2.4. Determination of Intra-Molecular Disulphide Bonds in CD9 and CD81 Tetraspanins
2.5. Molecular Modelling
3. Discussion
4. Materials and Methods
4.1. Primary Antibodies
4.2. Animals
4.3. Human Samples
4.4. Capacitation and Acrosome Reaction
4.4.1. Mouse Sperm
4.4.2. Human Sperm
4.5. Dual Immunofluorescent Staining of CD81 and CD9
4.6. Super-Resolution Microscopy
4.7. Protein Extraction
4.8. Co-Immunoprecipitation of CD9 and CD81 Molecules
4.9. SDS-PAGE with Immunoblotting
4.10. Molecular Modelling of CD9 and CD81 Tetraspanin Web
4.11. Data Analysis
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Frolikova, M.; Manaskova-Postlerova, P.; Cerny, J.; Jankovicova, J.; Simonik, O.; Pohlova, A.; Secova, P.; Antalikova, J.; Dvorakova-Hortova, K. CD9 and CD81 Interactions and Their Structural Modelling in Sperm Prior to Fertilization. Int. J. Mol. Sci. 2018, 19, 1236. https://doi.org/10.3390/ijms19041236
Frolikova M, Manaskova-Postlerova P, Cerny J, Jankovicova J, Simonik O, Pohlova A, Secova P, Antalikova J, Dvorakova-Hortova K. CD9 and CD81 Interactions and Their Structural Modelling in Sperm Prior to Fertilization. International Journal of Molecular Sciences. 2018; 19(4):1236. https://doi.org/10.3390/ijms19041236
Chicago/Turabian StyleFrolikova, Michaela, Pavla Manaskova-Postlerova, Jiri Cerny, Jana Jankovicova, Ondrej Simonik, Alzbeta Pohlova, Petra Secova, Jana Antalikova, and Katerina Dvorakova-Hortova. 2018. "CD9 and CD81 Interactions and Their Structural Modelling in Sperm Prior to Fertilization" International Journal of Molecular Sciences 19, no. 4: 1236. https://doi.org/10.3390/ijms19041236