Transcriptome Analysis Reveals Candidate Genes Associated with Leaf Etiolation of a Cytoplasmic Male Sterility Line in Chinese Cabbage (Brassica Rapa L. ssp. Pekinensis)
Abstract
:1. Introduction
2. Results
2.1. Phenotype and Ultrastructure of the Etiolated and Non-Etiolated Leaves
2.2. Content of Main Pigments and Photosynthetic Rate
2.3. Transcriptome Assembly and Annotation
2.4. Identification of Differentially Expressed Genes (DEGs)
2.5. Functional Classification of DEGs
3. Discussion
4. Materials and Methods
4.1. Plant Materials
4.2. Assays of the Content of Main Pigment
4.3. Determinations of Photosynthetic Rate
4.4. Transmission Electron Microscopy
4.5. RNA Extraction, cDNA Library Construction, and Sequencing
4.6. RNA-Sequencing Data Analysis
4.7. Quantitative Real-Time PCR Analysis
5. Conclusions
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Xie, F.; Yuan, J.-L.; Li, Y.-X.; Wang, C.-J.; Tang, H.-Y.; Xia, J.-H.; Yang, Q.-Y.; Wan, Z.-J. Transcriptome Analysis Reveals Candidate Genes Associated with Leaf Etiolation of a Cytoplasmic Male Sterility Line in Chinese Cabbage (Brassica Rapa L. ssp. Pekinensis). Int. J. Mol. Sci. 2018, 19, 922. https://doi.org/10.3390/ijms19040922
Xie F, Yuan J-L, Li Y-X, Wang C-J, Tang H-Y, Xia J-H, Yang Q-Y, Wan Z-J. Transcriptome Analysis Reveals Candidate Genes Associated with Leaf Etiolation of a Cytoplasmic Male Sterility Line in Chinese Cabbage (Brassica Rapa L. ssp. Pekinensis). International Journal of Molecular Sciences. 2018; 19(4):922. https://doi.org/10.3390/ijms19040922
Chicago/Turabian StyleXie, Fei, Jia-Lan Yuan, Yi-Xiao Li, Can-Jie Wang, Hong-Yu Tang, Jun-Hui Xia, Qing-Yong Yang, and Zheng-Jie Wan. 2018. "Transcriptome Analysis Reveals Candidate Genes Associated with Leaf Etiolation of a Cytoplasmic Male Sterility Line in Chinese Cabbage (Brassica Rapa L. ssp. Pekinensis)" International Journal of Molecular Sciences 19, no. 4: 922. https://doi.org/10.3390/ijms19040922