Functional Expression of TRP Ion Channels in Endometrial Stromal Cells of Endometriosis Patients
Abstract
:1. Introduction
2. Results
2.1. mRNA Expression Profile of TRP Channels in Endometrial Biospies and Primary hESC of Endometriosis Patients
2.2. Functional Expression of TRP Channels in Endometriosis-Derived hESC
2.3. Proliferative and Migratory Capacity of Endometriosis-Derived hESC
2.4. Eutopic vs. Ectopic
3. Discussion
4. Materials and Methods
4.1. Patients
4.2. Samples
4.2.1. Whole Endometrium Biopsies from Endometriosis Patients for TRP Expression Studies throughout the Menstrual Cycle
4.2.2. Primary Human Endometrial Stromal Cells
4.2.3. Whole Endometrium Biopsies and Endometriosis Lesions from Endometriosis Patients for Paired TRP Expression Study
4.3. RT–qPCR Experiments
4.3.1. Whole Endometrial Biopsies and Endometriotic Lesions
4.3.2. Primary Human Endometrial Stromal Cells
4.4. Functional Measurements
4.5. Proliferation Assay
4.6. Migration Assay
4.7. Data Analysis and Display
Supplementary Materials
Author Contributions
Funding
Acknowledgements
Conflicts of Interest
References
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Persoons, E.; Hennes, A.; De Clercq, K.; Van Bree, R.; Vriens, G.; O, D.F.; Peterse, D.; Vanhie, A.; Meuleman, C.; Voets, T.; et al. Functional Expression of TRP Ion Channels in Endometrial Stromal Cells of Endometriosis Patients. Int. J. Mol. Sci. 2018, 19, 2467. https://doi.org/10.3390/ijms19092467
Persoons E, Hennes A, De Clercq K, Van Bree R, Vriens G, O DF, Peterse D, Vanhie A, Meuleman C, Voets T, et al. Functional Expression of TRP Ion Channels in Endometrial Stromal Cells of Endometriosis Patients. International Journal of Molecular Sciences. 2018; 19(9):2467. https://doi.org/10.3390/ijms19092467
Chicago/Turabian StylePersoons, Eleonora, Aurélie Hennes, Katrien De Clercq, Rita Van Bree, Goede Vriens, Dorien F. O, Daniëlle Peterse, Arne Vanhie, Christel Meuleman, Thomas Voets, and et al. 2018. "Functional Expression of TRP Ion Channels in Endometrial Stromal Cells of Endometriosis Patients" International Journal of Molecular Sciences 19, no. 9: 2467. https://doi.org/10.3390/ijms19092467