Overexpression of a Wheat Aquaporin Gene, TdPIP2;1, Enhances Salt and Drought Tolerance in Transgenic Durum Wheat cv. Maali
Abstract
:1. Introduction
2. Results
2.1. Production of Transgenic Wheat TdPIP2;1 Plants
2.2. Analysis of GUS Activity in Relation to Stress Treatment
2.3. Evaluation of the Wheat TdPIP2;1 Gene for in Vitro Stress Tolerance
2.3.1. Effect of Salt Stress on Seed Germination
2.3.2. Effect of Salt and Osmotic Stresses on Root and Leaf Length
2.4. Evaluation of the Wheat TdPIP2;1 Gene for Stress Tolerance under Greenhouse Conditions
2.4.1. Fraction of Transpirable Soil Water (FTSW)
2.4.2. Effect on Biomass and Grain Production
2.5. Na+ and K+ Accumulation in Transgenic Wheat TdPIP2;1 Plants under Salt Stress Treatment
2.6. Oxidative Stress Evaluation of Transgenic Wheat TdPIP2;1 Plants
3. Discussion
4. Materials and methods
4.1. Plant Material
4.2. Construction of the Binary Vector and Wheat Transformation Procedure
4.3. Histochemical GUS Staining
4.4. GusA Expression Analysis by the Quantitative Real-Time qPCR
4.5. Evaluation of Transgenic Wheat Plants for Abiotic Stress Tolerance
4.5.1. In Vitro Assays
4.5.2. Greenhouse Assays
4.5.3. Water Treatments
4.5.4. Plant Transpiration Rate
4.6. Whole Plant Response Modelling to Increasing Water Deficit
y = 1;
else y = 1/FTSWt × FTSW;
y being NTR
4.7. Lipid Peroxidation
4.8. Quantitative H2O2 Measurement
4.9. Enzyme Assays
4.10. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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BP | (R2) | |
---|---|---|
WT | 0.363 | 0.871 |
SP3 | 0.909 | 0.823 |
PR3 | 0.983 | 0.656 |
PR6 | 0.803 | 0.838 |
Primers | Nucleotides Sequences (5′-3′) |
---|---|
Act F: | TGCATAGAGGGAAAGCACG |
Act R: | AACCCAAAAGCCAACAACAGAGA |
q-GusF: | CACGCCGTATGTTATTGCCG |
q-GusR: | TCTTGCCGTTTTCGTGGGTA |
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Ayadi, M.; Brini, F.; Masmoudi, K. Overexpression of a Wheat Aquaporin Gene, TdPIP2;1, Enhances Salt and Drought Tolerance in Transgenic Durum Wheat cv. Maali. Int. J. Mol. Sci. 2019, 20, 2389. https://doi.org/10.3390/ijms20102389
Ayadi M, Brini F, Masmoudi K. Overexpression of a Wheat Aquaporin Gene, TdPIP2;1, Enhances Salt and Drought Tolerance in Transgenic Durum Wheat cv. Maali. International Journal of Molecular Sciences. 2019; 20(10):2389. https://doi.org/10.3390/ijms20102389
Chicago/Turabian StyleAyadi, Malika, Faiçal Brini, and Khaled Masmoudi. 2019. "Overexpression of a Wheat Aquaporin Gene, TdPIP2;1, Enhances Salt and Drought Tolerance in Transgenic Durum Wheat cv. Maali" International Journal of Molecular Sciences 20, no. 10: 2389. https://doi.org/10.3390/ijms20102389