Spatiotemporal Expression of Anticoagulation Factor Antistasin in Freshwater Leeches
Abstract
:1. Introduction
2. Results and Discussion
2.1. Phylogeny of Antistasin Orthologs in Serine Protease Inhibitor Groups
2.2. Temporal Expression of Hau-Antistasin Genes during Embryogenesis
2.3. Spatial Expression of Hau-Antistasin during Organogenesis
2.4. Co-Linearity and Diversity of Antistasin Orthologs in Glossiphoniidae Leeches
2.5. Hau-Antistasin1 is Regulated by Hedgehog (hh) Signaling
3. Materials and Methods
3.1. Animals
3.2. Phylogenetic Analysis and Domain Prediction
3.3. Gene Identification, Gene Cloning, and Probe Synthesis
3.4. Developmental Semiquantitative RT-PCR
3.5. Whole-mount In Situ Hybridization and Cross Sectioning
3.6. Drug treatments and qPCR
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Kwak, H.-J.; Park, J.-S.; Medina Jiménez, B.I.; Park, S.C.; Cho, S.-J. Spatiotemporal Expression of Anticoagulation Factor Antistasin in Freshwater Leeches. Int. J. Mol. Sci. 2019, 20, 3994. https://doi.org/10.3390/ijms20163994
Kwak H-J, Park J-S, Medina Jiménez BI, Park SC, Cho S-J. Spatiotemporal Expression of Anticoagulation Factor Antistasin in Freshwater Leeches. International Journal of Molecular Sciences. 2019; 20(16):3994. https://doi.org/10.3390/ijms20163994
Chicago/Turabian StyleKwak, Hee-Jin, Jeong-Su Park, Brenda Irene Medina Jiménez, Soon Cheol Park, and Sung-Jin Cho. 2019. "Spatiotemporal Expression of Anticoagulation Factor Antistasin in Freshwater Leeches" International Journal of Molecular Sciences 20, no. 16: 3994. https://doi.org/10.3390/ijms20163994