Antithrombotic Effects of Paeoniflorin from Paeonia suffruticosa by Selective Inhibition on Shear Stress-Induced Platelet Aggregation
Abstract
:1. Introduction
2. Results
2.1. Paeoniflorin from Paeonia Suffruticosa Extract Significantly Inhibited Shear Stress-Induced Platelet Aggregation
2.2. Paeoniflorin Selectively Inhibits Platelet Aggregation-Induced by Shear Stress
2.3. Paeoniflorin Inhibits Platelet Activation Events in High Shear Stress Condition
2.4. Paeoniflorin Inhibited SIPA through Modulating vWF-GP Ib Interaction
2.5. Paeoniflorin Significantly Prevented Thrombus Formation without Increased Bleeding Risk
3. Discussion
4. Materials and Methods
4.1. Materials
4.2. Human Platelet Preparation
4.3. Measurement of Shear Stress-Induced Platelet Aggregation
4.4. Assessment of Cytoplasmic Calcium Levels
4.5. Assessment of P-Selectin Expression, GP IIb/IIIa Activation, and Fibrinogen Binding
4.6. Assessment of Platelet Cytotoxicity
4.7. Measurement of Agonist-Induced Platelet Aggregation in human PRP
4.8. Determination Effects on Thrombus Formation under Flow Conditions
4.9. Determination of Serotonin Release
4.10. Ex Vivo Determination of Anti-SIPA Activity
4.11. In Vivo Arterial Thrombosis Model
4.12. In Vivo Tail Bleeding Time Measurement
4.13. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Conflicts of Interest
Abbreviations
Ab | antibody |
ADP | adenosine diphosphate |
AM | acetoxymethyl ester |
aPTT | activated partial thromboplastin time |
CLO | clopidogrel |
DMSO | dimethyl sulfoxide |
FITC | fluorescein isothiocyanate |
GP | glycoprotein |
LDH | lactate dehydrogenase |
PBS | phosphate-buffered saline |
PE | phycoerythrin |
PF | paeoniflorin |
PGE1 | prostaglandin E1 |
PRP | platelet-rich plasma |
WP | washed platelet |
PT | prothrombin time |
SIPA | shear stress-induced platelet aggregation |
vWF | von Willebrand factor |
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Ngo, T.; Kim, K.; Bian, Y.; Noh, H.; Lim, K.-M.; Chung, J.-H.; Bae, O.-N. Antithrombotic Effects of Paeoniflorin from Paeonia suffruticosa by Selective Inhibition on Shear Stress-Induced Platelet Aggregation. Int. J. Mol. Sci. 2019, 20, 5040. https://doi.org/10.3390/ijms20205040
Ngo T, Kim K, Bian Y, Noh H, Lim K-M, Chung J-H, Bae O-N. Antithrombotic Effects of Paeoniflorin from Paeonia suffruticosa by Selective Inhibition on Shear Stress-Induced Platelet Aggregation. International Journal of Molecular Sciences. 2019; 20(20):5040. https://doi.org/10.3390/ijms20205040
Chicago/Turabian StyleNgo, Thien, Keunyoung Kim, Yiying Bian, Hakjun Noh, Kyung-Min Lim, Jin-Ho Chung, and Ok-Nam Bae. 2019. "Antithrombotic Effects of Paeoniflorin from Paeonia suffruticosa by Selective Inhibition on Shear Stress-Induced Platelet Aggregation" International Journal of Molecular Sciences 20, no. 20: 5040. https://doi.org/10.3390/ijms20205040