Relationship between Microsatellite Instability, Immune Cells Infiltration, and Expression of Immune Checkpoint Molecules in Ovarian Carcinoma: Immunotherapeutic Strategies for the Future
Abstract
:1. Introduction
2. Results
2.1. Clinicopathological Factors
2.2. Microsatellite Instability
2.3. Relationship between MSI and Expression of CD8, PD-L1, and PD-1
2.4. MSI Analysis
3. Discussion
4. Materials and Methods
4.1. Ethics Statement
4.2. Tissue Samples
4.3. Immunohistochemistry
4.4. Microsatellite Instability Analysis
4.5. Statistical Analyses
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
Abbreviations
FIGO | International Federation of Gynecology and Obstetrics |
MSI | microsatellite instability |
MMR | mismatch repair |
OS | overall survival |
PFS | progression-free survival |
PD-1 | programmed cell death-1 |
PD-L1 | programmed cell death-ligand 1 |
PDS | primary debulking surgery |
NAC | neoadjuvant chemotherapy |
IDS | interval debulking surgery |
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Characteristic | MSI | MSS | p-Value |
---|---|---|---|
n = 6 | n = 130 | ||
Age: number (%) | 0.496 | ||
<60 | 3 (50) | 54 (42) | |
≥60 | 3 (50) | 76 (58) | |
FIGO Stage: number (%) | 0.357 | ||
I, II | 3 (50) | 45 (35) | |
III, IV | 3 (50) | 85 (65) | |
Initial treatment (%) | 0.419 | ||
PDS | 6 (100) | 112 (86) | |
NAC | 0 (0) | 18 (14) | |
Residual tumor after PDS or IDS (%) | 0.202 | ||
No residual tumor (R0) | 4 (67) | 53 (41) | |
Yes | 2 (33) | 77 (59) |
Parameter | MSI | MSS | p-Value |
---|---|---|---|
n = 6 | n = 130 | ||
CD8: number (%) | 0.126 | ||
Positive | 5 (83) | 66 (51) | |
Negative | 1 (17) | 64 (49) |
Parameter | MSI | MSS | p-Value |
---|---|---|---|
n = 6 | n = 130 | ||
PD-L1: number (%) | 0.432 | ||
Positive | 2 (33) | 30 (23) | |
Negative | 4 (67) | 100 (77) |
Parameter | MSI | MSS | p-Value |
---|---|---|---|
n = 6 | n = 130 | ||
PD-1: number (%) | 0.653 | ||
Positive | 0 (0) | 9 (7) | |
Negative | 6 (100) | 121 (93) |
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Yamashita, H.; Nakayama, K.; Ishikawa, M.; Ishibashi, T.; Nakamura, K.; Sawada, K.; Yoshimura, Y.; Tatsumi, N.; Kurose, S.; Minamoto, T.; et al. Relationship between Microsatellite Instability, Immune Cells Infiltration, and Expression of Immune Checkpoint Molecules in Ovarian Carcinoma: Immunotherapeutic Strategies for the Future. Int. J. Mol. Sci. 2019, 20, 5129. https://doi.org/10.3390/ijms20205129
Yamashita H, Nakayama K, Ishikawa M, Ishibashi T, Nakamura K, Sawada K, Yoshimura Y, Tatsumi N, Kurose S, Minamoto T, et al. Relationship between Microsatellite Instability, Immune Cells Infiltration, and Expression of Immune Checkpoint Molecules in Ovarian Carcinoma: Immunotherapeutic Strategies for the Future. International Journal of Molecular Sciences. 2019; 20(20):5129. https://doi.org/10.3390/ijms20205129
Chicago/Turabian StyleYamashita, Hitomi, Kentaro Nakayama, Masako Ishikawa, Tomoka Ishibashi, Kohei Nakamura, Kiyoka Sawada, Yuki Yoshimura, Nagisa Tatsumi, Sonomi Kurose, Toshiko Minamoto, and et al. 2019. "Relationship between Microsatellite Instability, Immune Cells Infiltration, and Expression of Immune Checkpoint Molecules in Ovarian Carcinoma: Immunotherapeutic Strategies for the Future" International Journal of Molecular Sciences 20, no. 20: 5129. https://doi.org/10.3390/ijms20205129