Carboxypeptidase E-∆N Promotes Proliferation and Invasion of Pancreatic Cancer Cells via Upregulation of CXCR2 Gene Expression
Abstract
:1. Introduction
2. Results
2.1. CPE Transcripts and Proteins Expressed in Human Pancreatic Cancer Cell Lines
2.2. Subcellular Distribution of CPE and CPE-ΔN in Panc-1 Cells
2.3. Overexpression of CPE-ΔN in Panc-1 Cells Increases Proliferation and Invasion
2.4. CPE-ΔN Upregulates CXCR2 Expression in Panc-1 Cells
2.5. CPE-ΔN Induces Proliferation of Panc-1 Cells in a CXCR2-Dependent Manner
3. Discussion
4. Materials and Methods
4.1. Cell Lines
4.2. Northern Blot
4.3. Transfection of Cells with CPE-ΔN and CPE-WT Constructs
4.4. Immunoprecipitation of CPE/CPE-ΔN
4.5. Western Blot
4.6. Immunocytochemistry
4.7. Quantitative Real-Time RT-PCR
4.8. MTT Cell Proliferation Assay
4.9. Invasion Assay
4.10. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Abbreviations
CPE | Carboxypeptidase E |
PC | Pancreatic cancer |
HCC | Hepatocellular carcinoma |
WT | Wild-type |
PDAC | Pancreatic ductal adenocarcinoma |
OS | Osteosarcoma |
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Hareendran, S.; Yang, X.; Lou, H.; Xiao, L.; Loh, Y.P. Carboxypeptidase E-∆N Promotes Proliferation and Invasion of Pancreatic Cancer Cells via Upregulation of CXCR2 Gene Expression. Int. J. Mol. Sci. 2019, 20, 5725. https://doi.org/10.3390/ijms20225725
Hareendran S, Yang X, Lou H, Xiao L, Loh YP. Carboxypeptidase E-∆N Promotes Proliferation and Invasion of Pancreatic Cancer Cells via Upregulation of CXCR2 Gene Expression. International Journal of Molecular Sciences. 2019; 20(22):5725. https://doi.org/10.3390/ijms20225725
Chicago/Turabian StyleHareendran, Sangeetha, Xuyu Yang, Hong Lou, Lan Xiao, and Y. Peng Loh. 2019. "Carboxypeptidase E-∆N Promotes Proliferation and Invasion of Pancreatic Cancer Cells via Upregulation of CXCR2 Gene Expression" International Journal of Molecular Sciences 20, no. 22: 5725. https://doi.org/10.3390/ijms20225725