Platelet Proteasome Activity and Metabolism Is Upregulated during Bacterial Sepsis
Abstract
:1. Introduction
2. Results
2.1. Platelet Proteasome Activity and Protein Metabolism is Increased in the Septic Milieu
2.2. E. coli Exotoxin α-Hemolysin (hlyA) may be a Contributing Factor to Increased Proteasome Activity in Platelets
2.3. Platelet Proteasome Activity is Increased in Platelets from Sepsis Patients
2.4. Clinical Characteristics of Sepsis Patients
3. Discussion
4. Materials and Methods
4.1. Materials
4.2. Bacteria
4.3. Platelet Isolation and Preparation
4.4. RNA Isolation
4.5. Detection of Platelet Proteasome Activity
4.6. Quantification of Polyubiquitinated Protein in Human Platelets
4.7. Immunoblotting
4.8. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Clinical Characteristics of Seven Patients with Bacterial Sepsis | |
---|---|
Age | 63 [IQR 49; 69] |
Leukocyte count (1000/µL) | 16 [IQR 11.0; 21.0] |
CRP level (mg/dl) | 25 [IQR 19.5; 28.5] |
Procalcitonin (ng/mL) | 5.5 [IQR 4.0; 9.8] |
Positive bacterial culture | 7 of 7 |
Antibiotic treatment | 7 of 7 |
SOFA score | 9 [IQR 6; 11] |
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Grundler Groterhorst, K.; Mannell, H.; Pircher, J.; Kraemer, B.F. Platelet Proteasome Activity and Metabolism Is Upregulated during Bacterial Sepsis. Int. J. Mol. Sci. 2019, 20, 5961. https://doi.org/10.3390/ijms20235961
Grundler Groterhorst K, Mannell H, Pircher J, Kraemer BF. Platelet Proteasome Activity and Metabolism Is Upregulated during Bacterial Sepsis. International Journal of Molecular Sciences. 2019; 20(23):5961. https://doi.org/10.3390/ijms20235961
Chicago/Turabian StyleGrundler Groterhorst, Katharina, Hanna Mannell, Joachim Pircher, and Bjoern F Kraemer. 2019. "Platelet Proteasome Activity and Metabolism Is Upregulated during Bacterial Sepsis" International Journal of Molecular Sciences 20, no. 23: 5961. https://doi.org/10.3390/ijms20235961