Identification of QTL Related to ROS Formation under Hypoxia and Their Association with Waterlogging and Salt Tolerance in Barley
Abstract
:1. Introduction
2. Results
2.1. ROS (O2•−, H2O2) Production in Barley Cultivars under Hypoxia Stress
2.2. ROS Production in DH Lines and Identification of QTL for ROS Tolerance
2.3. Contribution of ROS (O2•−, H2O2) to Waterlogging and Salinity Tolerance
2.4. Effects of Using Waterlogging and Salt Tolerance As Covariates on QTL for ROS (O2•−, H2O2)
3. Discussion
4. Materials and Methods
4.1. Plant Material
4.2. Evaluation of the DH Lines for Waterlogging and Salinity Tolerance
4.3. Determination of Hydrogen Peroxide and Superoxide Radical for QTL
4.4. Genetic Map Construction and QTL Analysis
4.5. Statistical Analysis
Author Contributions
Acknowledgments
Conflicts of Interest
References
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Cultivar | O2•− Elongation Zone | O2•− Mature Zone | H2O2 Elongation Zone | H2O2 Mature Zone |
---|---|---|---|---|
TX9425 | 149 ± 7b | 189 ± 7b | 290 ± 14c | 400 ± 17b |
Naso Nijo | 197 ± 10a | 278 ± 16a | 515 ± 19a | 691 ± 18a |
DH lines | 194 ± 14a | 212 ± 28b | 416 ± 42b | 576 ± 73a |
DH lines range | 137–232 | 135–287 | 287–561 | 358–777 |
Trait | Linkage Group | Nearest Marker | Position (cM) | LOD | R2 (%) | Co-Variate |
---|---|---|---|---|---|---|
O2•− mature zone | 2H | 3271162D2 | 13.6 | 8.7 | 23.7 | |
No QTL identified | waterlogging | |||||
No QTL identified | salt | |||||
H2O2 elongation zone | 2H | 3999753D2 | 13.6 | 8.9 | 24.1 | |
No QTL identified | waterlogging | |||||
No QTL identified | salt | |||||
Waterlogging tolerance | 2H | 3264866S2 | 9.2 | 7.6 | 21 | |
2H | 3264866S2 | 9.2 | 5.6 | 14.8 | O2 | |
2H | 3264866S2 | 9.2 | 5.4 | 14.3 | H2O2 | |
Salt tolerance | 2H | 3257177S2 | 7.8 | 32.7 | 63.7 | |
2H | 3257177S2 | 7.8 | 26.7 | 39.4 | O2 | |
2H | 3257177S2 | 7.8 | 26.6 | 41.3 | H2O2 |
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Gill, M.B.; Zeng, F.; Shabala, L.; Zhang, G.; Yu, M.; Demidchik, V.; Shabala, S.; Zhou, M. Identification of QTL Related to ROS Formation under Hypoxia and Their Association with Waterlogging and Salt Tolerance in Barley. Int. J. Mol. Sci. 2019, 20, 699. https://doi.org/10.3390/ijms20030699
Gill MB, Zeng F, Shabala L, Zhang G, Yu M, Demidchik V, Shabala S, Zhou M. Identification of QTL Related to ROS Formation under Hypoxia and Their Association with Waterlogging and Salt Tolerance in Barley. International Journal of Molecular Sciences. 2019; 20(3):699. https://doi.org/10.3390/ijms20030699
Chicago/Turabian StyleGill, Muhammad Bilal, Fanrong Zeng, Lana Shabala, Guoping Zhang, Min Yu, Vadim Demidchik, Sergey Shabala, and Meixue Zhou. 2019. "Identification of QTL Related to ROS Formation under Hypoxia and Their Association with Waterlogging and Salt Tolerance in Barley" International Journal of Molecular Sciences 20, no. 3: 699. https://doi.org/10.3390/ijms20030699