Bisphenol A Activates Calcium Influx in Immortalized GnRH Neurons
Abstract
:1. Introduction
2. Results
2.1. Effects of BPA on Proliferation of GT1–7 Cells
2.2. BPA Does Not Affect GnRH Expression
2.3. BPA Induces Changes in [Ca2+]i
2.4. Changes in [Ca2+]i Induced by E2
3. Discussion
4. Materials and Methods
4.1. Materials
4.2. Cell Culture
4.3. Survival and Proliferation Assays
4.4. GnRH Expression Analysis
4.5. Calcium Imaging
4.6. Statistical Analysis
Author Contributions
Funding
Conflicts of Interest
References
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Ruffinatti, F.A.; Gilardino, A.; Secchi, V.; Cottone, E.; Lovisolo, D.; Bovolin, P. Bisphenol A Activates Calcium Influx in Immortalized GnRH Neurons. Int. J. Mol. Sci. 2019, 20, 2160. https://doi.org/10.3390/ijms20092160
Ruffinatti FA, Gilardino A, Secchi V, Cottone E, Lovisolo D, Bovolin P. Bisphenol A Activates Calcium Influx in Immortalized GnRH Neurons. International Journal of Molecular Sciences. 2019; 20(9):2160. https://doi.org/10.3390/ijms20092160
Chicago/Turabian StyleRuffinatti, Federico Alessandro, Alessandra Gilardino, Valter Secchi, Erika Cottone, Davide Lovisolo, and Patrizia Bovolin. 2019. "Bisphenol A Activates Calcium Influx in Immortalized GnRH Neurons" International Journal of Molecular Sciences 20, no. 9: 2160. https://doi.org/10.3390/ijms20092160