Hsp22 with an N-Terminal Domain Truncation Mediates a Reduction in Tau Protein Levels
Abstract
:1. Introduction
2. Results
2.1. Hsp22 Expression with Age and in AD
2.2. Hsp22 Prevents the Formation of Tau Aggregates In Vitro
2.3. Hsp22 NTDΔ Exacerbates Tau P301L Aggregate Formation In Vitro
2.4. Hsp22 NTDΔ Enhances the Clearance of Tau Protein in HEK293T Cells
2.5. Hsp22 WT and Phosphorylation Site Mutants Enhance the Clearance of Tau Protein in HEK293T Cells at High sHsp Ratios
3. Discussion
4. Materials and Methods
4.1. Hsp22 mRNA Expression in Human Brain Tissue
4.2. Transgenic Animal Brain Tissue Preparation
4.3. Anti-Hsp22 Immunohistochemistry Analysis
4.4. Analysis of Intrinsic Disorder Predisposition
4.5. Molecular Cloning
4.6. Protein Expression and Purification
4.7. Thioflavin T Fluorescence Assay
4.8. Transmission Electron Microscopy
4.9. Differential Scanning Fluorimetry
4.10. HEK 293T Co-Transfection and Fractionation
4.11. Western Blots
4.12. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Webster, J.M.; Darling, A.L.; Sanders, T.A.; Blazier, D.M.; Vidal-Aguiar, Y.; Beaulieu-Abdelahad, D.; Plemmons, D.G.; Hill, S.E.; Uversky, V.N.; Bickford, P.C.; et al. Hsp22 with an N-Terminal Domain Truncation Mediates a Reduction in Tau Protein Levels. Int. J. Mol. Sci. 2020, 21, 5442. https://doi.org/10.3390/ijms21155442
Webster JM, Darling AL, Sanders TA, Blazier DM, Vidal-Aguiar Y, Beaulieu-Abdelahad D, Plemmons DG, Hill SE, Uversky VN, Bickford PC, et al. Hsp22 with an N-Terminal Domain Truncation Mediates a Reduction in Tau Protein Levels. International Journal of Molecular Sciences. 2020; 21(15):5442. https://doi.org/10.3390/ijms21155442
Chicago/Turabian StyleWebster, Jack M., April L. Darling, Taylor A. Sanders, Danielle M. Blazier, Yamile Vidal-Aguiar, David Beaulieu-Abdelahad, Drew G. Plemmons, Shannon E. Hill, Vladimir N. Uversky, Paula C. Bickford, and et al. 2020. "Hsp22 with an N-Terminal Domain Truncation Mediates a Reduction in Tau Protein Levels" International Journal of Molecular Sciences 21, no. 15: 5442. https://doi.org/10.3390/ijms21155442