The Function of Oncogene B-Cell Lymphoma 6 in the Regulation of the Migration and Invasion of Trophoblastic Cells
Abstract
:1. Introduction
2. Results
2.1. Abundance of BCL6 in First Trimester Placentas Required for the Migration of Trophoblastic Cells
2.2. Suppression of BCL6 Reduces the Motility and Invasiveness of EVT Cells
2.3. BCL6 Depletion Reduces Cell Adhesion and Increases Filamentous Actin in HTR Cells
2.4. BCL6 Influences Multiple Signaling Pathways and Its Reduction is Associated with A Stationary Phenotype of HTR Cells
2.5. Reduced p-Paxilin and p-FAK Upon the Depletion or Inhibition of BCL6
2.6. Knockdown of BCL6 Interferes with the Expression of Pro-Migratory Genes
3. Discussion
4. Materials and Methods
4.1. Cell Culture, siRNA Transfection, Plasmid Cloning, Transfection, and Western Blot
4.2. Indirect Immunofluorescence Staining, Immunohistochemistry of Placental Tissue, and Intensity Measurement
4.3. Cell Adhesion, Migration, and Invasion Assay
4.4. Cell Motility Assay
4.5. RNA Extraction, Real-Time PCR, and Transcriptomic Analysis
4.6. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Abbreviations
PE | Preeclampsia |
BCL6 | B-cell lymphoma |
siRNA | small interfering RNA |
FA | Focal adhesion |
FAK | Focal adhesion kinase |
vCTB | villous trophoblastic cells |
EVTs | extravillous trophoblasts |
STBs | syncytiotrophoblasts |
HIPEC | HIPEC-65 |
HTR | HTR-8/SVneo |
PI3K | phosphoinositide 3-kinase |
Akt | Proteinkinasen B (PKBα/β/γ) |
Erk1/2 | extracellular regulated kinase 1/2 |
MAPK | mitogen-activated protein kinase |
STAT3 | signal transducer and activator of transcription 3 |
PAK1 | p21 RAC1 activated kinase 1 |
ACTG1 | actin gamma |
MYLK | myosin light chain kinase |
CAV1 | caveolin 1 |
MMP2 | matrix metalloproteinase-2 |
CDH1 | epithelial cadherin |
SSH1 | protein phosphatase Slingshot homolog 1 |
RPS6KA | ribosomal protein S6 kinase alpha-1 |
DUSP16 | dual specificity protein phosphatase 16 |
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Ritter, A.; Safdar, B.K.; Jasmer, B.; Kreis, N.-N.; Friemel, A.; Roth, S.; Solbach, C.; Louwen, F.; Yuan, J. The Function of Oncogene B-Cell Lymphoma 6 in the Regulation of the Migration and Invasion of Trophoblastic Cells. Int. J. Mol. Sci. 2020, 21, 8393. https://doi.org/10.3390/ijms21218393
Ritter A, Safdar BK, Jasmer B, Kreis N-N, Friemel A, Roth S, Solbach C, Louwen F, Yuan J. The Function of Oncogene B-Cell Lymphoma 6 in the Regulation of the Migration and Invasion of Trophoblastic Cells. International Journal of Molecular Sciences. 2020; 21(21):8393. https://doi.org/10.3390/ijms21218393
Chicago/Turabian StyleRitter, Andreas, Babek Khan Safdar, Britta Jasmer, Nina-Naomi Kreis, Alexandra Friemel, Susanne Roth, Christine Solbach, Frank Louwen, and Juping Yuan. 2020. "The Function of Oncogene B-Cell Lymphoma 6 in the Regulation of the Migration and Invasion of Trophoblastic Cells" International Journal of Molecular Sciences 21, no. 21: 8393. https://doi.org/10.3390/ijms21218393