Treatment with Gold Nanoparticles Using Cudrania tricuspidata Root Extract Induced Downregulation of MMP-2/-9 and PLD1 and Inhibited the Invasiveness of Human U87 Glioblastoma Cells
Abstract
:1. Introduction
2. Results
2.1. Facile and Green Synthesis of CTR-GNPs
2.2. Physicochemical Characterization of CTR-GNPs
2.3. CTR-GNPs Inhibit Human U87 Glioblastoma Cell Migration and Invasion
2.4. CTR-GNPs Inhibit MMP-2/-9 and PLD Activity in Human U87 Glioblastoma Cells
2.5. CTR-GNPs Inhibit MMP-2/-9 and PLD1 mRNA and Protein Expression in Human U87 Glioblastoma Cells
3. Discussion
4. Materials and Methods
4.1. Reagents
4.2. Preparation of Cudrania tricuspidata Root (CTR) Extract
4.3. Synthesis and Physicochemical Characterization of CTR-GNPs
4.4. Photocatalytic Activities of CTR-GNPs
4.5. Cell Culture and Cell Viability
4.6. Cell Transwell Migration and Invasion Assay
4.7. Gelatinase Zymography
4.8. Determination of PLD Activity
4.9. Total RNA Extraction and Quantitative Real Time PCR Analysis
4.10. Protein Preparation and Western Blotting Analysis
4.11. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Park, S.Y.; Cui, Z.; Kim, B.; Park, G.; Choi, Y.-W. Treatment with Gold Nanoparticles Using Cudrania tricuspidata Root Extract Induced Downregulation of MMP-2/-9 and PLD1 and Inhibited the Invasiveness of Human U87 Glioblastoma Cells. Int. J. Mol. Sci. 2020, 21, 1282. https://doi.org/10.3390/ijms21041282
Park SY, Cui Z, Kim B, Park G, Choi Y-W. Treatment with Gold Nanoparticles Using Cudrania tricuspidata Root Extract Induced Downregulation of MMP-2/-9 and PLD1 and Inhibited the Invasiveness of Human U87 Glioblastoma Cells. International Journal of Molecular Sciences. 2020; 21(4):1282. https://doi.org/10.3390/ijms21041282
Chicago/Turabian StylePark, Sun Young, Zhengwei Cui, Beomjin Kim, Geuntae Park, and Young-Whan Choi. 2020. "Treatment with Gold Nanoparticles Using Cudrania tricuspidata Root Extract Induced Downregulation of MMP-2/-9 and PLD1 and Inhibited the Invasiveness of Human U87 Glioblastoma Cells" International Journal of Molecular Sciences 21, no. 4: 1282. https://doi.org/10.3390/ijms21041282