Mechanisms and Regulation of Nonsense-Mediated mRNA Decay and Nonsense-Associated Altered Splicing in Lymphocytes
, Nivine Srour †, Laurent Delpy *,‡ and Jérôme Saulière ‡Round 1
Reviewer 1 Report
The manuscript described to PTCs-dependent RNA metabolism pathways. First is NMD to ensure an accelerated degration of mRNA s using NMD factors such as UPF proteins. Another pathway is NAS to upregulate transcripts that have skipped offending PTCs. The authors detailed and correctively presented the figures and manuscript to make even the general reader understand the complicated NMD and NAS field.
Author Response
Dear Reviewer 1,
We are grateful to your interest in our study and acknowledge all your comments.
As suggested, we have made several amendments to the text (lanes 64, 68, 103, 188, 214, 230) to improve the English language and style.
Best regards,
Laurent Delpy
Reviewer 2 Report
A review article submitted by Lambert et al focuses on the important aspect of RNA regulation in adaptive immune cells. This comprehensive review surely deserves to be published after considering the minor suggestions as follow:
It seems that authors have discussed more in relation to immunoglobulin diversity rather than the TCR recombination process. A little more focus on TCR mRNA regulation would be an added advantage. Authors are suggested to consider if they want to use "lymphoid" word in the title while focusing on B cells and T cells only, as lymphoid cells also include NK cells, Innate Lymphoid Cells. Figures should be high resolution, but I think this will be addressed subsequently.Author Response
Dear Reviewer 2,
We would first like to thank reviewer 2 for their supportive comments.
Point-by-point response:
- Authors are suggested to consider if they want to use "lymphoid" word in the title while focusing on B cells and T cells only, as lymphoid cells also include NK cells, Innate Lymphoid Cells...
We apologize for this and thank the reviewer for this pertinent comment. "Lymphoid cells" has now been replaced by "lymphocytes" in the title and at the end of the summary (lane 23).
- A little more focus on TCR mRNA regulation would be an added advantage...
We have added one sentence and 2 references on the impact of NMD inhibition on the development of T lymphocytes in mice. (lanes 132 and 133; ref. Frischmeyer-Guerrerio et al, PNAS 2011 Jun 28;108(26):10638-43. doi:10.1073/pnas.1019352108 and Weischenfeldt et al, Genes Dev. 2008 May 15;22(10):1381-96. doi: 10.1101/gad.468808.)
- Figures should be high resolution...
Better quality figures will be provided.
We thank you very much for giving us the opportunity to improve the quality of this review.
Best regards,
Laurent Delpy
