Synergistic Upregulation of Target Genes by TET1 and VP64 in the dCas9–SunTag Platform
Abstract
:1. Introduction
2. Results
2.1. Selection of Another Factor that Activates Gene Expression Synergistically with TET1 in the Modified dCas9–SunTag Format
2.2. Comparison of Synergistic Effects between Modified SunTag and dCas9 Direct Fusion
3. Discussion
4. Materials and Methods
4.1. Construction of sgRNAs
4.2. Cell Culture
4.3. Quantitative RT-PCR Analysis
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
Abbreviations
AAV | adeno-associated virus |
TALEs | transcription activator-like effectors |
dCas9 | catalytically dead Cas9 |
sgRNAs | single-guide RNAs |
SAM | synergistic activation mediator |
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Morita, S.; Horii, T.; Kimura, M.; Hatada, I. Synergistic Upregulation of Target Genes by TET1 and VP64 in the dCas9–SunTag Platform. Int. J. Mol. Sci. 2020, 21, 1574. https://doi.org/10.3390/ijms21051574
Morita S, Horii T, Kimura M, Hatada I. Synergistic Upregulation of Target Genes by TET1 and VP64 in the dCas9–SunTag Platform. International Journal of Molecular Sciences. 2020; 21(5):1574. https://doi.org/10.3390/ijms21051574
Chicago/Turabian StyleMorita, Sumiyo, Takuro Horii, Mika Kimura, and Izuho Hatada. 2020. "Synergistic Upregulation of Target Genes by TET1 and VP64 in the dCas9–SunTag Platform" International Journal of Molecular Sciences 21, no. 5: 1574. https://doi.org/10.3390/ijms21051574