Functional Analysis of Steroidogenic Factor 1 (sf-1) and 17α-Hydroxylase/Lyase (cyp17α) Promoters in Yellow Catfish Pelteobagrus fulvidraco
Abstract
:1. Introduction
2. Results
2.1. Cloning and Sequence Analysis of sf-1 and cyp17α Promoters
2.2. The Relative Luciferase Activity of 5’-Deletion Assay of the Regions of sf-1 and cyp17α Promoters
2.3. Overexpression Analysis of Yellow Catfish PPARα, PPARγ, and STAT3 of the Regions of sf-1 and cyp17α Promoters in HEK293T Cells
2.4. Site-Mutation Analysis of PPARα, PPARγ, and STAT3 on the Promoters of sf-1 and cyp17α
2.5. EMSA Analysis of Binding Sequence of Transcription Factors
3. Discussion
4. Materials and Methods
4.1. Experimental Animals and Reagents
4.2. Promoter Cloning and Plasmids Construction
4.3. Sequence Analysis
4.4. Plasmid Transfections and Assays of Luciferase Activities
4.5. Protein Expression of Yellow Catfish PPARα, PPARγ, and STAT3 in HEK293T Cells
4.6. Site-Mutation Analysis of Binding Sites on the sf-1 and cyp17α Promoters
4.7. Electrophoretic Mobility-Shift Assay (EMSA)
4.8. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Lv, W.-H.; Chen, G.-H.; Zhuo, M.-Q.; Xu, Y.-H.; Xu, Y.-C.; Tan, X.-Y. Functional Analysis of Steroidogenic Factor 1 (sf-1) and 17α-Hydroxylase/Lyase (cyp17α) Promoters in Yellow Catfish Pelteobagrus fulvidraco. Int. J. Mol. Sci. 2021, 22, 195. https://doi.org/10.3390/ijms22010195
Lv W-H, Chen G-H, Zhuo M-Q, Xu Y-H, Xu Y-C, Tan X-Y. Functional Analysis of Steroidogenic Factor 1 (sf-1) and 17α-Hydroxylase/Lyase (cyp17α) Promoters in Yellow Catfish Pelteobagrus fulvidraco. International Journal of Molecular Sciences. 2021; 22(1):195. https://doi.org/10.3390/ijms22010195
Chicago/Turabian StyleLv, Wu-Hong, Guang-Hui Chen, Mei-Qin Zhuo, Yi-Huan Xu, Yi-Chuang Xu, and Xiao-Ying Tan. 2021. "Functional Analysis of Steroidogenic Factor 1 (sf-1) and 17α-Hydroxylase/Lyase (cyp17α) Promoters in Yellow Catfish Pelteobagrus fulvidraco" International Journal of Molecular Sciences 22, no. 1: 195. https://doi.org/10.3390/ijms22010195