Cuticular Hydrocarbon Plasticity in Three Rice Planthopper Species
Abstract
:1. Introduction
2. Results
2.1. Effects of Planthopper Species, Developmental Stages and Sexes on Cuticular Hydrocarbon Profiles
2.2. Effects of Temperature and Relative Humidity on Cuticular Hydrocarbon Profiles
2.3. Effects of Different Host Plants on Cuticular Hydrocarbon Profiles
2.4. Effects of Temperature and Relative Humidity on Differential Gene Expression in Three Planthopper Species
2.5. Different Host Plant Effects on Differential Gene Expression in SBPH and WBPH
2.6. GO Enrichment Analysis
2.7. Cuticular Lipid Biosynthetic Pathway Gene Expression Profiles in the Three Species
3. Discussion
3.1. Exploring Factors Associated with Variation in CHC Profiles among the Planthopper Species
3.2. Searching for Clues Linking CHC Variation to Different Environmental Factors in the RNA-Seq Analysis
4. Materials and Methods
4.1. Maintenance of Planthopper Strains
4.2. Set-Up of Thermal and Humid Experiments
4.3. Extraction and Quantification of CHCs
4.4. Statistical and Quantitative Analyses
4.5. cDNA Library Preparation and Illumina Sequencing
4.6. Transcriptome and Differential Expression Analysis
4.7. GO Enrichment Analysis of Differentially Expressed Genes
4.8. Expression Profile Analysis of Cuticular Lipid Biosynthetic Pathway Genes
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Li, D.-T.; Pei, X.-J.; Ye, Y.-X.; Wang, X.-Q.; Wang, Z.-C.; Chen, N.; Liu, T.-X.; Fan, Y.-L.; Zhang, C.-X. Cuticular Hydrocarbon Plasticity in Three Rice Planthopper Species. Int. J. Mol. Sci. 2021, 22, 7733. https://doi.org/10.3390/ijms22147733
Li D-T, Pei X-J, Ye Y-X, Wang X-Q, Wang Z-C, Chen N, Liu T-X, Fan Y-L, Zhang C-X. Cuticular Hydrocarbon Plasticity in Three Rice Planthopper Species. International Journal of Molecular Sciences. 2021; 22(14):7733. https://doi.org/10.3390/ijms22147733
Chicago/Turabian StyleLi, Dan-Ting, Xiao-Jin Pei, Yu-Xuan Ye, Xin-Qiu Wang, Zhe-Chao Wang, Nan Chen, Tong-Xian Liu, Yong-Liang Fan, and Chuan-Xi Zhang. 2021. "Cuticular Hydrocarbon Plasticity in Three Rice Planthopper Species" International Journal of Molecular Sciences 22, no. 14: 7733. https://doi.org/10.3390/ijms22147733