Subunits of the PBAP Chromatin Remodeler Are Capable of Mediating Enhancer-Driven Transcription in Drosophila
Abstract
:1. Introduction
2. Results
2.1. In Vivo Targeting of SAYP or BAP170 to a Minimal Promoter Mediates Enhancer-Dependent Transcriptional Activation
2.2. Promoter-Bound PBAP Subunit Functions as a Tethering Factor for the hsp70 Minimal Promoter
2.3. Tethered BAP170/SAYP Facilitates Capture of Distant Enhancers
2.4. Tethered PBAP Subunit Recruits the Whole Complex onto the hsp70 Promoter
2.5. tSAYP-Dependent Reporter Transcription Crucially Depends on BAP170 and the Core PBAP Subunits
2.6. tBAP170-Driven Transcriptional Activation Is Resistant to Depletion of SAYP and PBAP Core Subunits
3. Discussion
4. Materials and Methods
4.1. Plasmid Preparation
4.2. Beta-Gal Staining, In Situ Hybridization, and Immunofluorescence
4.3. Transgenic Line Preparation
4.4. Drosophila Strains
4.5. Antibodies
4.6. Polytene Chromosome Analysis
4.7. Chromatin Immunoprecipitation, 3C and FAIRE Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Shidlovskii, Y.V.; Bylino, O.V.; Shaposhnikov, A.V.; Kachaev, Z.M.; Lebedeva, L.A.; Kolesnik, V.V.; Amendola, D.; De Simone, G.; Formicola, N.; Schedl, P.; et al. Subunits of the PBAP Chromatin Remodeler Are Capable of Mediating Enhancer-Driven Transcription in Drosophila. Int. J. Mol. Sci. 2021, 22, 2856. https://doi.org/10.3390/ijms22062856
Shidlovskii YV, Bylino OV, Shaposhnikov AV, Kachaev ZM, Lebedeva LA, Kolesnik VV, Amendola D, De Simone G, Formicola N, Schedl P, et al. Subunits of the PBAP Chromatin Remodeler Are Capable of Mediating Enhancer-Driven Transcription in Drosophila. International Journal of Molecular Sciences. 2021; 22(6):2856. https://doi.org/10.3390/ijms22062856
Chicago/Turabian StyleShidlovskii, Yulii V., Oleg V. Bylino, Alexander V. Shaposhnikov, Zaur M. Kachaev, Lyubov A. Lebedeva, Valeria V. Kolesnik, Diego Amendola, Giovanna De Simone, Nadia Formicola, Paul Schedl, and et al. 2021. "Subunits of the PBAP Chromatin Remodeler Are Capable of Mediating Enhancer-Driven Transcription in Drosophila" International Journal of Molecular Sciences 22, no. 6: 2856. https://doi.org/10.3390/ijms22062856