The Critical Role of NLRP6 Inflammasome in Streptococcus pneumoniae Infection In Vitro and In Vivo
Abstract
:1. Introduction
2. Results
2.1. NLRP6 Inflammasome Mediates Proinflammatory Cytokines Secretion during Macrophages Infection with S. pneumoniae
2.2. NLRP6 Is Involved in the Maturation and Secretion of IL-1β But Not in the Induction of IL-1β Transcription in S. pneumoniae-Infected Macrophages
2.3. NLRP6 Inflammasome Mediates Activation of Caspase-1 and Caspase-11 in S. pneumoniae-Infected Macrophages
2.4. NLRP6 Negatively Regulates NF-κB and ERK Signal Pathways in S. pneumoniae-Infected Macrophages
2.5. NLRP6 Damages the Host in S. pneumoniae Infection
2.6. NLRP6 Regulates S. pneumoniae-Induced Immune Response In Vivo
3. Discussion
4. Materials and Methods
4.1. Mice
4.2. Bacteria
4.3. In Vivo Infection Experiments
4.4. Flow Cytometric Analysis
4.5. Macrophages
4.6. ELISA
4.7. Western Blot Analysis
4.8. ASC Oligomerization
4.9. Quantitative RT-PCR
4.10. Statistical Analysis
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Xu, D.; Wu, X.; Peng, L.; Chen, T.; Huang, Q.; Wang, Y.; Ye, C.; Peng, Y.; Hu, D.; Fang, R. The Critical Role of NLRP6 Inflammasome in Streptococcus pneumoniae Infection In Vitro and In Vivo. Int. J. Mol. Sci. 2021, 22, 3876. https://doi.org/10.3390/ijms22083876
Xu D, Wu X, Peng L, Chen T, Huang Q, Wang Y, Ye C, Peng Y, Hu D, Fang R. The Critical Role of NLRP6 Inflammasome in Streptococcus pneumoniae Infection In Vitro and In Vivo. International Journal of Molecular Sciences. 2021; 22(8):3876. https://doi.org/10.3390/ijms22083876
Chicago/Turabian StyleXu, Dongyi, Xingping Wu, Lianci Peng, Tingting Chen, Qingyuan Huang, Yu Wang, Chao Ye, Yuanyi Peng, Dongliang Hu, and Rendong Fang. 2021. "The Critical Role of NLRP6 Inflammasome in Streptococcus pneumoniae Infection In Vitro and In Vivo" International Journal of Molecular Sciences 22, no. 8: 3876. https://doi.org/10.3390/ijms22083876