The HSV-1 Transcription Factor ICP4 Confers Liquid-Like Properties to Viral Replication Compartments
Abstract
:1. Introduction
2. Results
2.1. The HSV-1 Transcription Factor ICP4 Is a Viral IDP That Drives the Formation of Nuclear Condensates
2.2. ICP4 Condensates Have Liquid-Like Properties
2.3. The ICP4 NTA and DBD Control the Protein Phase Behavior
2.4. ICP4 Retains Its Liquid-Like Properties in HSV-1 VRCs
3. Discussion
4. Materials and Methods
4.1. Cells and Viruses
4.2. Plasmid and Amplicon Constructs
4.3. 3D Live-Cell Imaging
4.4. ICP4 Live-Cell Phase Diagrams
4.5. 1,6-Hexanediol Treatment
4.6. FRAP Experiments
4.7. ATP Depletion
4.8. Western Blot Analysis
4.9. Prediction Algorithms
4.10. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Seyffert, M.; Georgi, F.; Tobler, K.; Bourqui, L.; Anfossi, M.; Michaelsen, K.; Vogt, B.; Greber, U.F.; Fraefel, C. The HSV-1 Transcription Factor ICP4 Confers Liquid-Like Properties to Viral Replication Compartments. Int. J. Mol. Sci. 2021, 22, 4447. https://doi.org/10.3390/ijms22094447
Seyffert M, Georgi F, Tobler K, Bourqui L, Anfossi M, Michaelsen K, Vogt B, Greber UF, Fraefel C. The HSV-1 Transcription Factor ICP4 Confers Liquid-Like Properties to Viral Replication Compartments. International Journal of Molecular Sciences. 2021; 22(9):4447. https://doi.org/10.3390/ijms22094447
Chicago/Turabian StyleSeyffert, Michael, Fanny Georgi, Kurt Tobler, Laurent Bourqui, Michela Anfossi, Kevin Michaelsen, Bernd Vogt, Urs F. Greber, and Cornel Fraefel. 2021. "The HSV-1 Transcription Factor ICP4 Confers Liquid-Like Properties to Viral Replication Compartments" International Journal of Molecular Sciences 22, no. 9: 4447. https://doi.org/10.3390/ijms22094447