Selenium Antagonizes Cadmium-Induced Inflammation and Oxidative Stress via Suppressing the Interplay between NLRP3 Inflammasome and HMGB1/NF-κB Pathway in Duck Hepatocytes
Abstract
:1. Introduction
2. Results
2.1. Selection of Cd and Se Dosage
2.2. Se Alleviates Cd-Induced Hepatocytes Injury
2.3. Se Suppresses Cd-Stimulated Increases in ALT, AST and LDH Release from Hepatocytes
2.4. Se Relieves Cd-Induced Oxidative Stress and Inflammation of Hepatocyte
2.5. Se Represses Cd-Induced Activation of NLRP3 Inflammasomes
2.6. Se Curbs Cd-Caused Activation of HMGB1/NF-κB Signaling Pathway in Hepatocytes
2.7. HMGB1 Exerts a Vital Role in Se Restraining Cd-Induced Oxidative Stress and Inflammation of Hepatocytes
3. Discussion
4. Materials and Methods
4.1. Cells Isolation and Cells Culture
4.2. Cells Viability Assay
4.3. Determination of LDH Release
4.4. Assay of Transaminase Index
4.5. Measurement of Antioxidant Function Index
4.6. Detection of Intracellular ROS
4.7. Immunofluorescence Staining
4.8. Colocalization Analysis of NLRP3 and ASC
4.9. Preparation of Culture Supernatant
4.10. Enzyme-Linked Immunosorbent Measure (ELISA)
4.11. Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR)
4.12. Western Blotting Analyses
4.13. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Gene Name | Accession Number | Primer Sequences (5′ to 3′) |
---|---|---|
HMGB1 | XM_027469875.2 | Forward:AGTGTGAGGAGGCTGCGTAT Reverse: TAGACCTTTGGGGCCGTGTG |
TLR4 | NM_001310413.1 | Forward:CACCAGTTTCACTTCCCCTTGT Reverse: GCTTTGCTAGGGATGACTCCAA |
MYD88 | NM_001310832.1 | Forward:GCTTATAGAAAGGAGGTGTCGG Reverse: TGAAAGTCGCATTCGTCGCT |
TNF-α | XM_005027491.5 | Forward: GCATTTCGTTTTCCTTTTCAACT Reverse: ACCGTCTGAACTGTAACGGG |
IL-1β | XM_038166869.1 | Forward: TGGGCATCAAGGGCTACA Reverse: TCGGGTTGGTTGGTGATG |
caspase-1 | XM_038165654.1 | Forward: CACTGCAAGGCACTGATTGG Reverse: CCAGGAGACGGTATCTCCAC |
NLRP3 | XM_005029958.4 | Forward:CCAGCCTGAAGATCGGAGACCT Reverse: AGGAGCCACCCTAGAGGAGAGT |
β-actin | NM_001310421.1 | Forward:CAGCACGATGAAAATCAAGATCA Reverse: CAAGGGTGTGGGTGTTGGTAA |
IL-6 | XM_027450925.2 | Forward:TGGCTTCGACGAGGAGAAATG Reverse: CGTCGTTGCCAGATGCTTTG |
ASC | XM_013201308. 1 | Forward: CAGCATTCTGGATCGGCTCT Reverse: ATTTTCTCCTGCCTGATGCTT |
IL-18 | XM_027444356.2 | Forward: CCTGAAATCCCCTCCCGCTA Reverse: AGCTCATCTTCACCCTCGGT |
NF-κB | XM_005017679.4 | Forward:ACAACGTCCTTCATTTAGCAA Reverse: TCTGATAAAGGTCGTTCCTCA |
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Cao, Z.; Yang, F.; Lin, Y.; Shan, J.; Cao, H.; Zhang, C.; Zhuang, Y.; Xing, C.; Hu, G. Selenium Antagonizes Cadmium-Induced Inflammation and Oxidative Stress via Suppressing the Interplay between NLRP3 Inflammasome and HMGB1/NF-κB Pathway in Duck Hepatocytes. Int. J. Mol. Sci. 2022, 23, 6252. https://doi.org/10.3390/ijms23116252
Cao Z, Yang F, Lin Y, Shan J, Cao H, Zhang C, Zhuang Y, Xing C, Hu G. Selenium Antagonizes Cadmium-Induced Inflammation and Oxidative Stress via Suppressing the Interplay between NLRP3 Inflammasome and HMGB1/NF-κB Pathway in Duck Hepatocytes. International Journal of Molecular Sciences. 2022; 23(11):6252. https://doi.org/10.3390/ijms23116252
Chicago/Turabian StyleCao, Zhanyou, Fan Yang, Yiqun Lin, Jiyi Shan, Huabin Cao, Caiying Zhang, Yu Zhuang, Chenghong Xing, and Guoliang Hu. 2022. "Selenium Antagonizes Cadmium-Induced Inflammation and Oxidative Stress via Suppressing the Interplay between NLRP3 Inflammasome and HMGB1/NF-κB Pathway in Duck Hepatocytes" International Journal of Molecular Sciences 23, no. 11: 6252. https://doi.org/10.3390/ijms23116252