High-Mobility Group Box 1 Inhibitor BoxA Alleviates Neuroinflammation-Induced Retinal Ganglion Cell Damage in Traumatic Optic Neuropathy
Abstract
:1. Introduction
2. Results
2.1. HMGB1 Is Released in Retina after ONC
2.2. Optimizing the Dosage of BoxA for Intravitreal Injection
2.3. Effects of BoxA in RGCs Function after ONC
2.4. Inhibition of HMGB1 Protects Retinal Structure and Reduces RGCs Apoptosis in Experimental ONC
2.5. HMGB1 Inhibitor BoxA Inhibits the Activation of Retinal Microglia
2.6. HMGB1 Mediates NLRP3 Inflammasome, NF-kB after ONC
3. Discussion
3.1. Protection of RGCs by BOXA in Traumatic Optic Neuropathy
3.2. Possible Mechanisms of BoxA Mediates the Neuroinflammation-Induced Damage
4. Materials and Methods
4.1. Animals
4.2. Study Design
4.3. Establishment of Optic Nerve Crush (ONC) Model
4.4. Pattern Electroretinogram (P-ERG)
4.5. Scotopic Threshold Responses (STR)
4.6. Optical Coherence Tomography (OCT)
4.7. Immunofluorescence
4.8. Flat-Mounted Retinas and RGC Quantification
4.9. Automated Western Immunoblotting
4.10. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Treatment | Mean Amplitude of ERG Waves ± SD (µv) | Thickness ± SD (µm) | |||
---|---|---|---|---|---|
N2 | pSTR | ||||
Control | 11.44 ± 1.33 | 51.07 ± 6.20 | 79.87 ± 7.46 | ||
ONC + PBS | 6 d | 4.47 ± 0.83 | 7 d | 17.23 ± 3.46 | 72.95 ± 3.95 |
13 d | 1.72 ± 0.73 | 14 d | 2.23 ± 0.61 | 57.14 ± 3.58 | |
ONC + BoxA | 6 d | 7.54 ± 1.14 | 7 d | 31.88 ± 4.09 | 73.69 ± 4.91 |
13 d | 4.50 ± 1.14 | 14 d | 17.08 ± 2.93 | 64.66 ± 1.85 |
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Peng, J.; Jin, J.; Su, W.; Shao, W.; Li, W.; Li, Z.; Yu, H.; Zheng, Y.; Zhong, L. High-Mobility Group Box 1 Inhibitor BoxA Alleviates Neuroinflammation-Induced Retinal Ganglion Cell Damage in Traumatic Optic Neuropathy. Int. J. Mol. Sci. 2022, 23, 6715. https://doi.org/10.3390/ijms23126715
Peng J, Jin J, Su W, Shao W, Li W, Li Z, Yu H, Zheng Y, Zhong L. High-Mobility Group Box 1 Inhibitor BoxA Alleviates Neuroinflammation-Induced Retinal Ganglion Cell Damage in Traumatic Optic Neuropathy. International Journal of Molecular Sciences. 2022; 23(12):6715. https://doi.org/10.3390/ijms23126715
Chicago/Turabian StylePeng, Jingyi, Jiayi Jin, Wenru Su, Wanwen Shao, Weihua Li, Zhiquan Li, Huan Yu, Yongxin Zheng, and Liuxueying Zhong. 2022. "High-Mobility Group Box 1 Inhibitor BoxA Alleviates Neuroinflammation-Induced Retinal Ganglion Cell Damage in Traumatic Optic Neuropathy" International Journal of Molecular Sciences 23, no. 12: 6715. https://doi.org/10.3390/ijms23126715