MicroRNA-381 Regulates Proliferation and Differentiation of Caprine Skeletal Muscle Satellite Cells by Targeting PTEN and JAG2
Abstract
:1. Introduction
2. Results
2.1. Isolation, Identification and Myogenic Differentiation of Caprine SMSCs
2.2. Expression Profiles of MyoG, MyHC and miR-381 during Myogenic Differentiation
2.3. miR-381 Accelerates Myogenic Differentiation of Caprine SMSCs
2.4. miR-381 Suppresses Viability and Proliferation of Caprine SMSCs
2.5. Prediction and KEGG Pathway Analysis of the Target Genes of miR-381
2.6. Validation of Target Relationships of miR-381 with the Target Genes
3. Discussion
4. Materials and Methods
4.1. Ethics Statement
4.2. Isolation, Identification and Myogenic Differentiation of Caprine SMSCs
4.3. The Effect of miR-381 on Caprine SMSC Differentiation
4.4. The Effect of miR-381 on the Viability and Proliferation of Caprine SMSCs
4.5. Prediction and Validation of the Target Genes of miR-381
4.6. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Shen, J.; Wang, J.; Zhen, H.; Liu, Y.; Li, L.; Luo, Y.; Hu, J.; Liu, X.; Li, S.; Hao, Z.; et al. MicroRNA-381 Regulates Proliferation and Differentiation of Caprine Skeletal Muscle Satellite Cells by Targeting PTEN and JAG2. Int. J. Mol. Sci. 2022, 23, 13587. https://doi.org/10.3390/ijms232113587
Shen J, Wang J, Zhen H, Liu Y, Li L, Luo Y, Hu J, Liu X, Li S, Hao Z, et al. MicroRNA-381 Regulates Proliferation and Differentiation of Caprine Skeletal Muscle Satellite Cells by Targeting PTEN and JAG2. International Journal of Molecular Sciences. 2022; 23(21):13587. https://doi.org/10.3390/ijms232113587
Chicago/Turabian StyleShen, Jiyuan, Jiqing Wang, Huimin Zhen, Yan Liu, Lu Li, Yuzhu Luo, Jiang Hu, Xiu Liu, Shaobin Li, Zhiyun Hao, and et al. 2022. "MicroRNA-381 Regulates Proliferation and Differentiation of Caprine Skeletal Muscle Satellite Cells by Targeting PTEN and JAG2" International Journal of Molecular Sciences 23, no. 21: 13587. https://doi.org/10.3390/ijms232113587