GmWRI1c Increases Palmitic Acid Content to Regulate Seed Oil Content and Nodulation in Soybean (Glycine max)
Abstract
:1. Introduction
2. Results
2.1. Identification of WRI Gene Family Members in Soybean
2.2. Conserved Function of GmWRI1c in Fatty Acid Synthesis
2.3. GmWRI1c Specifically Increases Palmitic Acid Content by Regulating Genes Related to Glycolysis and De Novo Lipogenesis
2.4. Overexpression of GmWRI1c Promotes Nodulation Genes’ Expression and Increases Nodule Numbers in Soybean
2.5. Superior Allele of GmWRI1cHap1 Increases Seed Oil Content and Nodule Numbers in Soybean
3. Discussion
4. Materials and Methods
4.1. Plant Materials and Growth Conditions
4.2. Identification and Bioinformatics Analysis of GmWRI1c
4.3. Subcellular Localisation Analysis
4.4. Generation of Transgenic Arabidopsis
4.5. RNA Extraction and cDNA Synthesis
4.6. Quantitative Real-Time PCR Analysis
4.7. Soybean Hairy Root Transformation and Nodulation Assay
4.8. Determination of Soluble Sugars Content, Total Fatty Acid Content, and Fatty Acid Composition
4.9. Determination of GUS Activity
4.10. Soybean Oil Content Determination
4.11. Tassel Analysis and Genotyping
4.12. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Abbreviations
Cleaved amplified polymorphic sequence | CAPS |
Coding sequence | CDS |
Haplotype | Hap |
Hidden Markov model | HMM |
Quantitative real-time PCR | qRT-PCR |
Recombinant inbred line | RIL |
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Zheng, H.; Zhao, D.; Shao, W.; Lu, Y.; Wang, W.; Hu, Y.; Li, J.; Zhu, S.; Wang, X. GmWRI1c Increases Palmitic Acid Content to Regulate Seed Oil Content and Nodulation in Soybean (Glycine max). Int. J. Mol. Sci. 2022, 23, 13793. https://doi.org/10.3390/ijms232213793
Zheng H, Zhao D, Shao W, Lu Y, Wang W, Hu Y, Li J, Zhu S, Wang X. GmWRI1c Increases Palmitic Acid Content to Regulate Seed Oil Content and Nodulation in Soybean (Glycine max). International Journal of Molecular Sciences. 2022; 23(22):13793. https://doi.org/10.3390/ijms232213793
Chicago/Turabian StyleZheng, Haowei, Duo Zhao, Wentao Shao, Yun Lu, Wenhui Wang, Yanjiao Hu, Jiajia Li, Shangshang Zhu, and Xiaobo Wang. 2022. "GmWRI1c Increases Palmitic Acid Content to Regulate Seed Oil Content and Nodulation in Soybean (Glycine max)" International Journal of Molecular Sciences 23, no. 22: 13793. https://doi.org/10.3390/ijms232213793