Rno_circ_0001004 Acts as a miR-709 Molecular Sponge to Regulate the Growth Hormone Synthesis and Cell Proliferation
Abstract
:1. Introduction
2. Results
2.1. Characterization of Rno_circ_0001004 in GH3 Cells
2.2. Rno_circ_0001004 Antagonizes miR-709-Mediated Repression of GH Synthesis and Secretion
2.3. Knockdown of Rno_circ_0001004 Suppresses the GH Synthesis and Secretion in GH3 Cell
2.4. Rno_circ_0001004 Promoted the Viability of GH3 Cells
2.5. Rno_circ_0001004 Reversed the Inhibition of Cell Proliferation by miR-709
3. Discussion
4. Materials and Methods
4.1. Cell Culture and Transfection
4.2. RAN Isolation, cDNA Synthesis, RT-PCR and Sanger Sequencing
4.3. Vector Construction
4.4. Dual-Luciferase Reporter Assay
4.5. Evaluation of GH3 Proliferation
4.6. Western Blot Analysis
4.7. Quantification of Secretory GH by ELISA
4.8. Statistics Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Xiong, J.; Zhang, H.; Wang, Y.; Cheng, Y.; Luo, J.; Chen, T.; Xi, Q.; Sun, J.; Zhang, Y. Rno_circ_0001004 Acts as a miR-709 Molecular Sponge to Regulate the Growth Hormone Synthesis and Cell Proliferation. Int. J. Mol. Sci. 2022, 23, 1413. https://doi.org/10.3390/ijms23031413
Xiong J, Zhang H, Wang Y, Cheng Y, Luo J, Chen T, Xi Q, Sun J, Zhang Y. Rno_circ_0001004 Acts as a miR-709 Molecular Sponge to Regulate the Growth Hormone Synthesis and Cell Proliferation. International Journal of Molecular Sciences. 2022; 23(3):1413. https://doi.org/10.3390/ijms23031413
Chicago/Turabian StyleXiong, Jiali, Haojie Zhang, Yuxuan Wang, Yunyun Cheng, Junyi Luo, Ting Chen, Qianyun Xi, Jiajie Sun, and Yongliang Zhang. 2022. "Rno_circ_0001004 Acts as a miR-709 Molecular Sponge to Regulate the Growth Hormone Synthesis and Cell Proliferation" International Journal of Molecular Sciences 23, no. 3: 1413. https://doi.org/10.3390/ijms23031413