Caveolin-1 Regulation and Function in Mouse Uterus during Early Pregnancy and under Human In Vitro Decidualization
Abstract
:1. Introduction
2. Results
2.1. Caveolin-1 Expression during Early Pregnancy
2.2. Caveolin-1 Expression under Delayed Implantation and Activation
2.3. Estrogen Regulation on Caveolin-1 Expression
2.4. Progesterone Regulation of Caveolin-1 Expression
2.5. Role of Caveolin-1 during Mouse In Vitro Decidualization
2.6. Caveolin-1 Expression under Human In Vitro Decidualization
2.7. Effects of Caveolin-1 Over-Expression on Human In Vitro Decidualization
2.8. Regulation of Caveolin-1 Expression
3. Discussion
3.1. Hormonal Regulation
3.2. Senescence
3.3. Decidualization
3.4. Embryonic Regulation
3.5. Insulin Regulation
4. Materials and Methods
4.1. Animals
4.2. Isolation of Mouse Uterine Endometrial Stromal Cells
4.3. Cell Culture and In Vitro Decidualization of Human Endometrial Stromal Cells
4.4. Caveolin-1 siRNA and Overexpression
4.5. Real-Time PCR
4.6. Western Blot Analysis
4.7. Immunofluorescence
4.8. SA-β-Gal Staining
4.9. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Abbreviations
CAV1 | Caveolin-1 |
COX2 | Cyclooxygenase-2 |
CREB | cAMP-response element-binding protein |
DPN | Diarylpropionitrile |
ERα | Estrogen receptor α |
hCG | human chorionic gonadotropin |
ICI | ICI 182780 (Fulvestrant) |
PPT | Estrogen receptor α agonist |
SA-β-Gal | Senescence-associated-β-galactosidase |
PR | Progesterone receptor |
PRL | Prolactin |
TNFα | Tumor necrosis factor α |
ZO-1 | Zonula occludens-1 |
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Gene | Species | Sequence (5′-3′) | Application | Accession Number | Product Size (bp) |
---|---|---|---|---|---|
Rpl7 | Mouse | GCAGATGTACCGCACTGAGATTC ACCTTTGGGCTTACTCCATTGATA | RT-qPCR | NM_011291.5 | 129 |
Prl8a2 | Mouse | AGCCAGAAATCACTGCCACT TGATCCATGCACCCATAAAA | RT-qPCR | NM_010088 | 119 |
Rpl19 | Mouse | TCATGGAGCACATCCACAAGCTGA CGCTTTCGTGCTTCCTTGGTCTTA | RT-qPCR | NM_001287738.1 | 197 |
Cav1 | Mouse | ATTCAGCAACATCCGCATCAG AGAGTGAGGACAGCAACCAAT | RT-qPCR | NM_001243064.1 | 494 |
IGFBP1 | Human | CCAAACTGCAACAAGAATG GTAGACGCACCAGCAGAG | RT-qPCR | NM_001013029 | 87 |
RPL7 | Human | CTGCTGTGCCAGAAACCCTT TCTTGCCATCCTCGCCAT | RT-qPCR | NM_000971 | 194 |
GAPDH | Human | GAAGGTGAAGGTCGGAGT GATGGCAACAATATCCACTT | RT-qPCR | BC023632 | 94 |
CAV1 | Human | GCATCAGCCGTGTCTATTCC GCAGTTGAGGTTGTTGGTTCT | RT-qPCR | NM_001172896.2 | 476 |
Cav1 | Mouse | CCACCTTCACTGTGACAAA | siRNA | ||
CAV1 | Human | GCCGUGUCUAUUCCAUCUA | siRNA | ||
NC | - | CTCCGAACGTGTCACGT | siRNA |
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Song, Z.; Li, B.; Li, M.-Y.; Luo, J.-M.; Hong, Y.-Q.; He, Y.-Y.; Chen, S.-T.; Yang, Z.-S.; Liang, C.; Yang, Z.-M. Caveolin-1 Regulation and Function in Mouse Uterus during Early Pregnancy and under Human In Vitro Decidualization. Int. J. Mol. Sci. 2022, 23, 3699. https://doi.org/10.3390/ijms23073699
Song Z, Li B, Li M-Y, Luo J-M, Hong Y-Q, He Y-Y, Chen S-T, Yang Z-S, Liang C, Yang Z-M. Caveolin-1 Regulation and Function in Mouse Uterus during Early Pregnancy and under Human In Vitro Decidualization. International Journal of Molecular Sciences. 2022; 23(7):3699. https://doi.org/10.3390/ijms23073699
Chicago/Turabian StyleSong, Zhuo, Bo Li, Meng-Yuan Li, Jia-Mei Luo, Yu-Qi Hong, Yu-Ying He, Si-Ting Chen, Zhen-Shan Yang, Chen Liang, and Zeng-Ming Yang. 2022. "Caveolin-1 Regulation and Function in Mouse Uterus during Early Pregnancy and under Human In Vitro Decidualization" International Journal of Molecular Sciences 23, no. 7: 3699. https://doi.org/10.3390/ijms23073699