Cyclophilin A Isomerisation of Septin 2 Mediates Abscission during Cytokinesis
Abstract
:1. Introduction
2. Results
2.1. Sept2 Is a Novel Interacting Partner of CypA
2.2. Localisation of Sept2 at the Midbody in Human Cancer Cells
2.3. Depletion of CypA Disrupts Sept2 Localisation at the Midbody
2.4. Inhibition of CypA Isomerase Activity Alters Sept2 Dynamics at the Midbody
2.5. Isomerisation of Pro259 Is Required for Central Midbody Localisation of Sept2
2.6. Structural and Molecular Analysis of CypA-Sept2P259A Interaction
2.7. Isomerisation of Sept2 Pro259 Is Required for the Completion of Cell Division
3. Discussion
4. Materials and Methods
4.1. Cell Culture, Synchronisation and Transfection
4.2. Proteomics Sample Preparation, Analysis and Identification of Sept2 as a CypA Interactor
4.3. Co-Immunoprecipitation
4.4. Immunoblotting
4.5. Immunofluorescence
4.6. Data and Image Analysis
4.7. Isomerase Assay
4.8. Plasmids and Site-Directed Mutagenesis (SDM)
4.9. Modelling of the Interaction between CypA and Sept2
4.10. Time-Lapse Spinning Disk Microscopy
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Conflicts of Interest
References
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Gorry, R.L.; Brennan, K.; Lavin, P.T.M.; Mazurski, T.; Mary, C.; Matallanas, D.; Guichou, J.-F.; Mc Gee, M.M. Cyclophilin A Isomerisation of Septin 2 Mediates Abscission during Cytokinesis. Int. J. Mol. Sci. 2023, 24, 11084. https://doi.org/10.3390/ijms241311084
Gorry RL, Brennan K, Lavin PTM, Mazurski T, Mary C, Matallanas D, Guichou J-F, Mc Gee MM. Cyclophilin A Isomerisation of Septin 2 Mediates Abscission during Cytokinesis. International Journal of Molecular Sciences. 2023; 24(13):11084. https://doi.org/10.3390/ijms241311084
Chicago/Turabian StyleGorry, Rebecca L., Kieran Brennan, Paul T. M. Lavin, Tayler Mazurski, Charline Mary, David Matallanas, Jean-François Guichou, and Margaret M. Mc Gee. 2023. "Cyclophilin A Isomerisation of Septin 2 Mediates Abscission during Cytokinesis" International Journal of Molecular Sciences 24, no. 13: 11084. https://doi.org/10.3390/ijms241311084